Document Detail


Practical issues in high-speed cell sorting.
MedLine Citation:
PMID:  20069524     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Modern flow cytometric cell sorters are all capable of so-called "high-speed sorting." However, there is confusion about exactly how fast a "high-speed" cell sorter can sort cells. There are many considerations in achieving the fastest sorting speed, as well as the highest quality sort results--cell recovery, purity, and functionality. This requires the same considerations required for "slow-speed sorting"; however, a more precise implementation is required for high-speed sorting. The modern cell sorters enable high-speed sorting because of advances in high-speed electronics and data processing. We discuss the practical considerations of high-speed sorting in terms of the theory and practical aspects of the mechanical and software components of sorting, statistics of sorting, cell preparation and viability, instrument setup, sort strategies, and biosafety.
Authors:
Larry W Arnold; Joanne Lannigan
Related Documents :
16815904 - Measurement of fret efficiency and ratio of donor to acceptor concentration in living c...
3369274 - Differentiation of adreno-chromaffin cells in the newborn rat, as detected by formaldeh...
18539294 - Multispectral imaging of hematopoietic cells: where flow meets morphology.
15720764 - Photon moment analysis in cells in the presence of photo-bleaching.
19185734 - Methods to investigate autophagy during starvation and regeneration in hydra.
19120694 - Abeta aggregation and possible implications in alzheimer's disease pathogenesis.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]     Volume:  Chapter 1     ISSN:  1934-9300     ISO Abbreviation:  Curr Protoc Cytom     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-13     Completed Date:  2010-03-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100899351     Medline TA:  Curr Protoc Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 1.24.1-30     Citation Subset:  IM    
Affiliation:
The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Flow Cytometry / instrumentation*,  methods*
Humans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Shaping the future of vocational rehabilitation of adults: eight fields of action as starting points...
Next Document:  Use of flow cytometric methods to quantify protein-protein interactions.