| PrP octarepeats region determined the interaction with caveolin-1 and phosphorylation of caveolin-1 and Fyn. | |
| | |
MedLine Citation:
|
PMID: 23283514 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Caveolin-1 is one of the major constituents of caveolae. Both Cav-1 and PrP are plasma membrane proteins, which show active capacities for molecular interactions with many other proteins or agents, including themselves. Using yeast two-hybrid system and immunoprecipitation, we reconfirmed the molecular interaction between human Cav-1 and PrP. With co-immunoprecipitation tests, PrP(C)-Cav-1 and PrP(Sc)-Cav-1 complexes were identified in the brain homogenates of normal and scrapie agent 263K-infected hamsters, respectively. Transient expression of wild-type PrP (PrP-PG5) in HEK293 cells did not change the situation of Cav-1 and subsequent signal transduction pathways, while cross-linking of the expressed PrP with specific antibody induced remarkable colocalization of PrP and Cav-1 on the plasma membrane and significant increases of phosphorylated Cav-1 and phosphorylated Fyn. With deleted and inserted PrP mutants within octarepeat region, we observed obvious octarepeat-associated phenomena, including lower binding capacity with Cav-1 in vitro, unable to co-localize with Cav-1 in the cells and to induce up-regulation of p-Cav-1 and p-Fyn when removal of octarepeats in the context of full-length PrP. Moreover, we found that treatment on HEK293 cells with fibrous form of recombinant PrP protein led to up-regulating the levels of p-Cav-1 and p-Fyn. Our data here provide strong evidence that octarepeats of PrP are critical for the interaction between PrP and Cav-1. Significant alterations in the cultured cells, either the distributions of PrP and Cav-1 morphologically or the up-regulations of p-Cav-1 and p-Fyn, induced by antibody-mediated cross-linking or fibrous forms of PrP may suggest a possible internalization process of PrP(Sc). |
| | |
Authors:
|
Qi Shi; Yuan-Yuan Jing; Shao-Bin Wang; Cao Chen; Han Sun; Yin Xu; Chen Gao; Jin Zhang; Chan Tian; Yan Guo; Ke Ren; Xiao-Ping Dong |
Related Documents
:
|
6604574 - Molecular and biological characterization of anticarcinogenic and tumor cell growth-inh... 23099844 - Lyoniresinol inhibits melanogenic activity through the induction of microphthalmia-asso... 21150304 - Mamp-responsive mapk cascades regulate phytoalexin biosynthesis. 22927394 - Protein cysteine phosphorylation of sara/mgra family transcriptional regulators mediate... 7583124 - Phosphatidylinositol transfer protein dictates the rate of inositol trisphosphate produ... 19647484 - P38 mitogen-activated protein kinase inhibition reduces inflammatory cytokines in a bra... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-3 |
Journal Detail:
|
Title: Medical microbiology and immunology Volume: - ISSN: 1432-1831 ISO Abbreviation: Med. Microbiol. Immunol. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0314524 Medline TA: Med Microbiol Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Chang-Bai Rd 155, Beijing, 102206, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: EuLoc: a web-server for accurately predict protein subcellular localization in eukaryotes by incorpo...
Next Document: Genome-wide identification of laccase gene family in three Phytophthora species.