Document Detail

Potentiation of proliferation of some but not all human colon carcinoma cell lines by immobilized hepatic asialoglycoprotein receptor 1.
MedLine Citation:
PMID:  19725223     Owner:  NLM     Status:  MEDLINE    
Twenty-three human colorectal carcinoma cell lines were examined for the binding of recombinant hepatic asialoglycoprotein receptor 1 (ASGR1), which is known to be exclusively expressed on hepatic parenchymal cells. The effects of the binding were assessed by adhesion to and proliferation on immobilized recombinant ASGR1. Recombinant ASGR1 bound strongly to six cell lines and moderately to 15 cell lines out of 23 lines tested, as shown by flow cytometric analysis. The first six cell lines (group A) also exhibited strong adherence to immobilized ASGR1, whereas 11 of the 15 cell lines of the second group (group B) showed significant adhesion with smaller enhancement by ASGR1 than the cell lines in group A. With a representative cell line (DLD-1 cells categorized in group B), a significant portion of the adhesion was inhibited by preincubation of ASGR1 with asialofetuin, a competitive inhibitor of the carbohydrate recognition by ASGR1. The growth rates of 13 cell lines (two of group A and 11 of group B) were significantly accelerated when they were cultured on immobilized recombinant ASGR1. The results indicate that ASGR is a potential organ-specific microenvironmental factor for colorectal carcinoma growth and metastasis formation in livers.
Jinbo Fang; Ryota Izawa; Laura Gomez-Santos; Suguru Ueno; Tomoya Sawaguchi; Katsuaki Usami; Yoshiaki Nodera; Hideyuki Takeuchi; Yoshimi Ohashi; Nobuaki Higashi; Tatsuro Irimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology research     Volume:  17     ISSN:  0965-0407     ISO Abbreviation:  Oncol. Res.     Publication Date:  2009  
Date Detail:
Created Date:  2009-09-03     Completed Date:  2009-09-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208097     Medline TA:  Oncol Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  437-45     Citation Subset:  IM    
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
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MeSH Terms
Asialoglycoprotein Receptor / metabolism*
Asialoglycoproteins / pharmacology
Cell Adhesion
Cell Proliferation*
Colonic Neoplasms / metabolism*,  pathology*
Flow Cytometry
Recombinant Proteins / genetics,  metabolism
Tumor Cells, Cultured
alpha-Fetoproteins / pharmacology
Reg. No./Substance:
0/Asialoglycoprotein Receptor; 0/Asialoglycoproteins; 0/Recombinant Proteins; 0/alpha-Fetoproteins; 0/asialofetuin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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