| Potentiation of proliferation of some but not all human colon carcinoma cell lines by immobilized hepatic asialoglycoprotein receptor 1. | |
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MedLine Citation:
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PMID: 19725223 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Twenty-three human colorectal carcinoma cell lines were examined for the binding of recombinant hepatic asialoglycoprotein receptor 1 (ASGR1), which is known to be exclusively expressed on hepatic parenchymal cells. The effects of the binding were assessed by adhesion to and proliferation on immobilized recombinant ASGR1. Recombinant ASGR1 bound strongly to six cell lines and moderately to 15 cell lines out of 23 lines tested, as shown by flow cytometric analysis. The first six cell lines (group A) also exhibited strong adherence to immobilized ASGR1, whereas 11 of the 15 cell lines of the second group (group B) showed significant adhesion with smaller enhancement by ASGR1 than the cell lines in group A. With a representative cell line (DLD-1 cells categorized in group B), a significant portion of the adhesion was inhibited by preincubation of ASGR1 with asialofetuin, a competitive inhibitor of the carbohydrate recognition by ASGR1. The growth rates of 13 cell lines (two of group A and 11 of group B) were significantly accelerated when they were cultured on immobilized recombinant ASGR1. The results indicate that ASGR is a potential organ-specific microenvironmental factor for colorectal carcinoma growth and metastasis formation in livers. |
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Authors:
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Jinbo Fang; Ryota Izawa; Laura Gomez-Santos; Suguru Ueno; Tomoya Sawaguchi; Katsuaki Usami; Yoshiaki Nodera; Hideyuki Takeuchi; Yoshimi Ohashi; Nobuaki Higashi; Tatsuro Irimura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncology research Volume: 17 ISSN: 0965-0407 ISO Abbreviation: Oncol. Res. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-09-03 Completed Date: 2009-09-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9208097 Medline TA: Oncol Res Country: United States |
Other Details:
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Languages: eng Pagination: 437-45 Citation Subset: IM |
Affiliation:
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Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Asialoglycoprotein Receptor
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metabolism* Asialoglycoproteins / pharmacology Cell Adhesion Cell Proliferation* Colonic Neoplasms / metabolism*, pathology* Flow Cytometry Humans Recombinant Proteins / genetics, metabolism Tumor Cells, Cultured alpha-Fetoproteins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Asialoglycoprotein Receptor; 0/Asialoglycoproteins; 0/Recombinant Proteins; 0/alpha-Fetoproteins; 0/asialofetuin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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