| Potentiation of mitomycin C cytotoxicity by glutathione depletion in a multi-drug resistant mouse leukemia cell line. | |
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MedLine Citation:
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PMID: 1451095 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was undertaken to elucidate the mechanism of cross-resistance to mitomycin C (MMC) in a multi-drug resistant mouse leukemia cell line (P388/R-84). The P388/R-84 cells were 6.2-fold more resistant to MMC as compared to the sensitive cells (P388/S). Since oxygen radical formation has been suggested to be important in MMC cytotoxicity, MMC-dependent peroxidation of membrane lipids (an index of oxygen radical formation) was compared in these cell lines. MMC-dependent lipid peroxidation was 2.56-fold higher using P388/S cell extract when compared to that of P388/R-84 cells. Depletion of cellular glutathione (GSH) levels in the resistant cells produced a 1.76-fold increase in MMC-dependent lipid peroxidation. MMC cytotoxicity was also increased significantly by GSH depletion in P388/R-84 cells. These results suggest that cellular resistance to MMC in P388/R-84 cells may, at least in part, be due to the reduced formation of oxyradicals in these cells. |
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Authors:
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B H Xu; S V Singh |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer letters Volume: 66 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1993-01-04 Completed Date: 1993-01-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 49-53 Citation Subset: IM |
Affiliation:
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Mercy Cancer Center, Mercy Hospital of Pittsburgh, PA 15219. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Drug Resistance Drug Screening Assays, Antitumor Drug Synergism Glutathione / deficiency*, metabolism Leukemia P388 / drug therapy*, metabolism Lipid Peroxidation / drug effects Mice Mitomycin / pharmacology*, toxicity Reactive Oxygen Species / metabolism Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA 50638/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 50-07-7/Mitomycin; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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