| Potentiation of (-)-epigallocatechin-3-gallate-induced apoptosis by bortezomib in multiple myeloma cells. | |
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MedLine Citation:
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PMID: 20011976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The green tea constituent, (-)-epigallocatechin-3-gallate (EGCG), has chemopreventive and anticancer effects. This is partially because of the selective ability of EGCG to induce apoptosis and death in cancer cells without affecting normal cells. In the present study, the activity of EGCG against the myeloma cell line, KM3, was examined. Our results demonstrated, for the first time, that the treatment of the KM3 cell line with EGCG inhibits cell proliferation and induces apoptosis, and there is a synergistic effect when EGCG and bortezomib are combined. Further experiments showed that this effect involves the NF-kappaB pathway. EGCG inhibits the expression of the P65 mRNA and P65/pP65 protein, meanwhile it downregulates pIkappaBalpha expression and upregulates IkappaBalpha expression. EGCG also activates caspase-3, -8, cleaved caspase-9, and poly-ADP-ribose polymerase (PARP) and subsequent apoptosis. These findings provided experimental evidence for efficacy of EGCG alone or in combination with bortezomib in multiple myeloma therapy. |
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Authors:
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Qing Wang; Juan Li; Jingli Gu; Beihui Huang; Ying Zhao; Dong Zheng; Yan Ding; Lijin Zeng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta biochimica et biophysica Sinica Volume: 41 ISSN: 1745-7270 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-03-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 1018-26 Citation Subset: IM |
Affiliation:
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Department of Hematology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis / drug effects* Blotting, Western Boronic Acids / pharmacology* Catechin / analogs & derivatives*, pharmacology Cell Line, Tumor Humans Multiple Myeloma / metabolism, pathology* Polymerase Chain Reaction Pyrazines / pharmacology* RNA, Messenger / genetics, metabolism Tea / chemistry* Transcription Factor RelA / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Boronic Acids; 0/Pyrazines; 0/RNA, Messenger; 0/Tea; 0/Transcription Factor RelA; 0/bortezomib; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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