Document Detail

Potentiation of early necrotic death of glucose-starved pheochromocytoma 12 cells by nerve growth factor.
MedLine Citation:
PMID:  10987143     Owner:  NLM     Status:  MEDLINE    
Recently suggested is an arguable hypothesis that neurotrophins can induce necrosis but suppress apoptosis of target cells in some pathological conditions. We examined this hypothesis by tracing the type of NGF-promoted cell death occurring in a hypoglycemic condition at various angles, such as kinetic analyses, histological examinations of membrane alterations, morphological observations in ultra-structural changes, and determinations of DNA fragmentation. Glucose-starved cell death consisted of two kinetically different stages, suggesting that it be mixed with early and delayed death. Several lines of evidence revealed that NGF prominently enhanced the early death with necrotic characters. By contrast, apoptotic characters of glucose-starved delayed death were not much affected by NGF. Nifedipine, a voltage-gated calcium channel blocker, could completely compensate for the enhancement of the early glucose-starved death by NGF. Interestingly, the NGF-promoted cell death was also blocked by cycloheximide that did not keep PC12 cells alive from glucose starvation. Therefore, all the data in this study suggest that NGF accelerates the early necrosis of glucose-starved cell death probably through the alterations of intracellular calcium ions and protein syntheses.
J H Hong; K C Hur; J M Chung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  10     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-12-12     Completed Date:  2001-01-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  KOREA (SOUTH)    
Other Details:
Languages:  eng     Pagination:  443-51     Citation Subset:  IM    
Department of Biology and Center for Cell Signaling Research, Ewha Womans University, Seoul, Korea.
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MeSH Terms
Calcium Channel Blockers / pharmacology
Cell Membrane / ultrastructure
Cell Nucleus / ultrastructure
Cycloheximide / pharmacology
DNA Fragmentation / drug effects
Glucose / metabolism*
Microscopy, Confocal
Microscopy, Electron
Nerve Growth Factor / pharmacology*
Neurons / metabolism,  pathology*,  ultrastructure
Nifedipine / pharmacology
PC12 Cells
Protein Biosynthesis / drug effects
Reg. No./Substance:
0/Calcium Channel Blockers; 21829-25-4/Nifedipine; 50-99-7/Glucose; 66-81-9/Cycloheximide; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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