Document Detail


Potentiation by a biscoclaurine alkaloid, cepharanthine, of the toxicity of conjugates of epidermal growth factor with Pseudomonas exotoxin in HeLa cells.
MedLine Citation:
PMID:  3257717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cepharanthine, a biscoclaurine alkaloid, causes an 8-fold enhancement of the cytotoxic effect of a conjugate of epidermal growth factor (EGF) coupled with Pseudomonas exotoxin in HeLa cells. Cepharanthine also potentiates the effect of Pseudomonas exotoxin. Cepharanthine does not affect the binding and uptake of 125I-EGF by HeLa cells, but it delays the release of radioactivity associated with 125I-EGF into the medium. Analysis by colloidal silica gradients using cell homogenates suggests that 125I-EGF accumulates in the lysosomes of cells treated with cepharanthine and that [3H]cepharanthine accumulates in lysosomes. The pH in HeLa cell lysosomes is 5.2, and cepharanthine does not significantly increase the pH. Electron microscopy shows an increased number of electron-dense bodies and dilated Golgi apparatus after cepharanthine treatment. Cepharanthine appears to accumulate in lysosomes, and it may delay degradation of EGF-Pseudomonas exotoxin in the cells as does 125I-EGF.
Authors:
N Shiraishi; T Shimada; Y Hagino; K Kohno; M Kobayashi; M Kuwano; S Akiyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  48     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-03-28     Completed Date:  1988-03-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1307-11     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Oita Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
ADP Ribose Transferases*
Alkaloids / pharmacokinetics,  pharmacology*
Bacterial Toxins*
Benzylisoquinolines
Cell Survival / drug effects
Drug Synergism
Epidermal Growth Factor / administration & dosage,  metabolism,  pharmacology*
Exotoxins / administration & dosage,  pharmacology*
Hela Cells / drug effects,  ultrastructure
Humans
Hydrogen-Ion Concentration
Lysosomes / drug effects
Tissue Distribution
Virulence Factors*
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Bacterial Toxins; 0/Benzylisoquinolines; 0/Exotoxins; 0/Virulence Factors; 481-49-2/cepharanthine; 62229-50-9/Epidermal Growth Factor; EC 2.4.2.-/ADP Ribose Transferases; EC 2.4.2.31/toxA protein, Pseudomonas aeruginosa

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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