Document Detail


Potentiation of epithelial innate host responses by intercellular communication.
MedLine Citation:
PMID:  21124989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The epithelium efficiently attracts immune cells upon infection despite the low number of pathogenic microbes and moderate levels of secreted chemokines per cell. Here we examined whether horizontal intercellular communication between cells may contribute to a coordinated response of the epithelium. Listeria monocytogenes infection, transfection, and microinjection of individual cells within a polarized intestinal epithelial cell layer were performed and activation was determined at the single cell level by fluorescence microscopy and flow cytometry. Surprisingly, chemokine production after L. monocytogenes infection was primarily observed in non-infected epithelial cells despite invasion-dependent cell activation. Whereas horizontal communication was independent of gap junction formation, cytokine secretion, ion fluxes, or nitric oxide synthesis, NADPH oxidase (Nox) 4-dependent oxygen radical formation was required and sufficient to induce indirect epithelial cell activation. This is the first report to describe epithelial cell-cell communication in response to innate immune activation. Epithelial communication facilitates a coordinated infectious host defence at the very early stage of microbial infection.
Authors:
Tamas Dolowschiak; Cécilia Chassin; Sanae Ben Mkaddem; Thilo M Fuchs; Siegfried Weiss; Alain Vandewalle; Mathias W Hornef
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Publication Detail:
Type:  Journal Article     Date:  2010-11-18
Journal Detail:
Title:  PLoS pathogens     Volume:  6     ISSN:  1553-7374     ISO Abbreviation:  PLoS Pathog.     Publication Date:  2010  
Date Detail:
Created Date:  2010-12-02     Completed Date:  2011-03-16     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101238921     Medline TA:  PLoS Pathog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1001194     Citation Subset:  IM    
Affiliation:
Hannover Medical School, Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Toxins / metabolism
Blotting, Western
Cell Adhesion
Cell Communication*
Cells, Cultured
Cytokines / metabolism
Epithelial Cells / microbiology*
Flow Cytometry
Fluorescent Antibody Technique
Heat-Shock Proteins / metabolism
Hemolysin Proteins / metabolism
Immunity, Innate
Immunoblotting
Immunoprecipitation
Intestine, Small / immunology*,  metabolism,  microbiology
Listeria monocytogenes / pathogenicity*
Listeriosis / immunology*,  microbiology,  pathology
Mice
NADPH Oxidase / genetics,  metabolism
Nitric Oxide / metabolism
RNA, Messenger / genetics
Reactive Oxygen Species / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Cytokines; 0/Heat-Shock Proteins; 0/Hemolysin Proteins; 0/RNA, Messenger; 0/Reactive Oxygen Species; 10102-43-9/Nitric Oxide; 72270-41-8/hlyA protein, Listeria monocytogenes; EC 1.6.-/Nox4 protein, mouse; EC 1.6.3.1/NADPH Oxidase
Comments/Corrections
Comment In:
Nat Rev Immunol. 2011 Jan;11(1):2   [PMID:  21218655 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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