Document Detail


Potential therapeutic strategy for oral squamous cell carcinoma by ErbB3-binding protein 1 gene transfer.
MedLine Citation:
PMID:  19937055     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: An ErbB3-binding protein 1 (Ebp1), was shown to be a potent tumor suppressor in breast and prostate cancer cells. We hypothesized that the inhibitory properties of the Ebp1 gene could be beneficial if ectopically expressed in oral squamous cell carcinoma (OSCC) cells. METHODS: One OSCC cell line Tca8113 was stably transfected with the complete Ebp1 cDNA or the vector control pcDNA3.1. The inhibitory effect was evaluated using in vitro proliferation assay, cell cycle distribution and anchorage-independent growth in soft agar as well as in vivo tumorigenicity. RESULTS: Stable gene transfer was verified by Western Blot analysis and reverse transcription RT-PCR. Following transfection of Ebp1, a significant reduction in cell proliferation and anchorage-independent growth in soft agar were observed. Ectopic expression of Ebp1 led to a change in cell cycle profile and most importantly, a strong decrease in tumorigenicity of human OSCC cell line in a xenograft mouse model. CONCLUSIONS: Ectopic expression of Ebp1 mediates multiple antitumor activities against OSCC, suggesting a potential of Ebp1-based novel therapy for clinical OSCC treatment.
Authors:
Xu Zhou; Wantao Chen; Yuexing Zhang; Jian Sun; Qing Wang; Youcheng Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-24
Journal Detail:
Title:  Journal of cancer research and clinical oncology     Volume:  136     ISSN:  1432-1335     ISO Abbreviation:  J. Cancer Res. Clin. Oncol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-04-21     Completed Date:  2010-04-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902060     Medline TA:  J Cancer Res Clin Oncol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  891-6     Citation Subset:  IM    
Affiliation:
Zhongshan Hospital, Fudan University, 200032 Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / genetics*
Antineoplastic Agents / therapeutic use*
Blotting, Western
Carcinoma, Squamous Cell / genetics,  therapy*
Cell Line, Tumor
Cell Proliferation
Gene Therapy / methods*
Gene Transfer Techniques
Humans
Plasmids
RNA-Binding Proteins / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Tongue Neoplasms / genetics,  therapy*
Transfection
Up-Regulation
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Antineoplastic Agents; 0/PA2G4 protein, human; 0/RNA-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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