| Potential sources of oxidative stress that induce postexercise proteinuria in rats. | |
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MedLine Citation:
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PMID: 18258803 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exercise-induced proteinuria is a common consequence of physical activity and is caused predominantly by alterations in renal hemodynamics. Although it has been shown that exercise-induced oxidative stress can also contribute to the occurrence of postexercise proteinuria, the sources of reactive oxygen species that promote it are unknown. We investigated the enzymes nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and xanthine oxidase (XO) as possible sources of oxidative stress in postexercise proteinuria. First, we evaluated the effect of blocking the NADPH oxidase enzyme on postexercise proteinuria. We found a significant increase in urinary protein level, kidney thiobarbituric acid-reactive substances (TBARS), and protein carbonyl content after exhaustive exercise, and NADPH oxidase activity was induced by exercise. Rats that were treated with an NADPH oxidase inhibitor for 4 days before exhaustive exercise showed no increase in kidney TBARS or protein carbonyl derivative level and no proteinuria or NADPH oxidase activation. In the next set of experiments, we investigated the effect of XO blockage on postexercise proteinuria. Oxypurinol, an XO inhibitor was administered to rats for 3 days before exercise. Although XO inhibition significantly decreased kidney TBARS levels and protein carbonyl content in exercised rats, the inhibition did not prevent exercise-induced proteinuria. However, plasma and kidney XO activity was not induced by exercise, but rather it was suppressed under oxypurinol treatment. These results suggest that increased NADPH oxidase activity induced by exhaustive exercise is an important source of elevated oxidative, stress during exercise, which contributes to the occurrence of postexercise proteinuria. |
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Authors:
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Günnur Koçer; Umit Kemal Sentürk; Oktay Kuru; Filiz Gündüz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-07 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 104 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-04-03 Completed Date: 2008-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1063-8 Citation Subset: IM |
Affiliation:
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Akdeniz Univ., Medical Faculty, Dept. of Physiology, Kampus, 07070 Antalya, Turkey. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Enzyme Inhibitors / pharmacology Female Kidney / metabolism NADPH Oxidase / metabolism Oxidative Stress / physiology* Oxypurinol / pharmacology Physical Conditioning, Animal / adverse effects*, physiology Protein Carbonylation / drug effects, physiology Proteinuria / etiology*, metabolism Rats Rats, Wistar Thiobarbituric Acid Reactive Substances / metabolism Xanthine Oxidase / antagonists & inhibitors, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Thiobarbituric Acid Reactive Substances; 2465-59-0/Oxypurinol; EC 1.17.3.2/Xanthine Oxidase; EC 1.6.3.1/NADPH Oxidase |
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