| Potential role for purple acid phosphatase in the dephosphorylation of wall proteins in tobacco cells. | |
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MedLine Citation:
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PMID: 20357138 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is not yet known whether dephosphorylation of proteins catalyzed by phosphatases occurs in the apoplastic space. In this study, we found that tobacco (Nicotiana tabacum) purple acid phosphatase could dephosphorylate the phosphoryl residues of three apoplastic proteins, two of which were identified as alpha-xylosidase and beta-glucosidase. The dephosphorylation and phosphorylation of recombinant alpha-xylosidase resulted in a decrease and an increase in its activity, respectively, when xyloglucan heptasaccharide was used as a substrate. Attempted overexpression of the tobacco purple acid phosphatase NtPAP12 in tobacco cells not only decreased the activity levels of the glycosidases but also increased levels of xyloglucan oligosaccharides and cello-oligosaccharides in the apoplast during the exponential phase. We suggest that purple acid phosphatase controls the activity of alpha-xylosidase and beta-glucosidase, which are responsible for the degradation of xyloglucan oligosaccharides and cello-oligosaccharides in the cell walls. |
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Authors:
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Rumi Kaida; Satoshi Serada; Naoko Norioka; Shigemi Norioka; Lutz Neumetzler; Markus Pauly; Javier Sampedro; Ignacio Zarra; Takahisa Hayashi; Takako S Kaneko |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-31 |
Journal Detail:
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Title: Plant physiology Volume: 153 ISSN: 1532-2548 ISO Abbreviation: Plant Physiol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-04 Completed Date: 2010-09-15 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 603-10 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biological Sciences, Japan Women's University, Tokyo 112-8681, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB017967; AB084124; NM105527 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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metabolism* Cell Wall / metabolism* Cells, Cultured Glucans / metabolism Glycoproteins / metabolism* Molecular Sequence Data Phosphorylation Plant Proteins / metabolism* Proteome / metabolism Tobacco / enzymology* Xylans / metabolism Xylosidases / metabolism beta-Glucosidase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glucans; 0/Glycoproteins; 0/Plant Proteins; 0/Proteome; 0/Xylans; 37294-28-3/xyloglucan; EC 3.1.3.-/purple acid phosphatase; EC 3.1.3.2/Acid Phosphatase; EC 3.2.1.-/Xylosidases; EC 3.2.1.21/beta-Glucosidase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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