Document Detail

Potential role for purple acid phosphatase in the dephosphorylation of wall proteins in tobacco cells.
MedLine Citation:
PMID:  20357138     Owner:  NLM     Status:  MEDLINE    
It is not yet known whether dephosphorylation of proteins catalyzed by phosphatases occurs in the apoplastic space. In this study, we found that tobacco (Nicotiana tabacum) purple acid phosphatase could dephosphorylate the phosphoryl residues of three apoplastic proteins, two of which were identified as alpha-xylosidase and beta-glucosidase. The dephosphorylation and phosphorylation of recombinant alpha-xylosidase resulted in a decrease and an increase in its activity, respectively, when xyloglucan heptasaccharide was used as a substrate. Attempted overexpression of the tobacco purple acid phosphatase NtPAP12 in tobacco cells not only decreased the activity levels of the glycosidases but also increased levels of xyloglucan oligosaccharides and cello-oligosaccharides in the apoplast during the exponential phase. We suggest that purple acid phosphatase controls the activity of alpha-xylosidase and beta-glucosidase, which are responsible for the degradation of xyloglucan oligosaccharides and cello-oligosaccharides in the cell walls.
Rumi Kaida; Satoshi Serada; Naoko Norioka; Shigemi Norioka; Lutz Neumetzler; Markus Pauly; Javier Sampedro; Ignacio Zarra; Takahisa Hayashi; Takako S Kaneko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-31
Journal Detail:
Title:  Plant physiology     Volume:  153     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-04     Completed Date:  2010-09-15     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  603-10     Citation Subset:  IM    
Department of Chemical and Biological Sciences, Japan Women's University, Tokyo 112-8681, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB017967;  AB084124;  NM105527
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MeSH Terms
Acid Phosphatase / metabolism*
Cell Wall / metabolism*
Cells, Cultured
Glucans / metabolism
Glycoproteins / metabolism*
Molecular Sequence Data
Plant Proteins / metabolism*
Proteome / metabolism
Tobacco / enzymology*
Xylans / metabolism
Xylosidases / metabolism
beta-Glucosidase / metabolism
Reg. No./Substance:
0/Glucans; 0/Glycoproteins; 0/Plant Proteins; 0/Proteome; 0/Xylans; 37294-28-3/xyloglucan; EC 3.1.3.-/purple acid phosphatase; EC Phosphatase; EC 3.2.1.-/Xylosidases; EC

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