| Potential role of protein kinase B in glucose transporter 4 translocation in adipocytes. | |
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MedLine Citation:
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PMID: 9112399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphatidylinositol 3-kinase (PI 3-kinase) activation promotes glucose transporter 4 (Glut 4) translocation in adipocytes. In this study, we demonstrate that protein kinase B, a serine/threonine kinase stimulated by PI 3-kinase, is activated by both insulin and okadaic acid in isolated adipocytes, in parallel with their effects on Glut 4 translocation. In 3T3-L1 adipocytes, platelet-derived growth factor activated PI 3-kinase as efficiently as insulin but was only half as potent as insulin in promoting protein kinase B (PKB) activation. To look for a potential role of PKB in Glut 4 translocation, adipocytes were transfected with a constitutively active PKB (Gag-PKB) together with an epitope tagged transporter (Glut 4 myc). Gag-PKB was associated with all membrane fractions, whereas the endogenous PKB was mostly cytosolic. Expression of Gag-PKB led to an increase in Glut 4 myc amount at the cell surface. Our results suggest that PKB could play a role in promoting Glut 4 appearance at the cell surface following exposure of adipocytes to insulin and okadaic acid stimulation. |
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Authors:
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J F Tanti; S Grillo; T Grémeaux; P J Coffer; E Van Obberghen; Y Le Marchand-Brustel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrinology Volume: 138 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1997 May |
Date Detail:
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Created Date: 1997-05-09 Completed Date: 1997-05-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2005-10 Citation Subset: AIM; IM |
Affiliation:
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INSERM U 145, Faculté de Médecine, Nice, France. tanti@unice.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase Adipocytes / metabolism* Animals Biological Transport Enzyme Activation / drug effects Glucose Transporter Type 4 Immunoblotting Immunosorbent Techniques Insulin / pharmacology Male Monosaccharide Transport Proteins / metabolism* Muscle Proteins* Okadaic Acid / pharmacology Phosphotransferases (Alcohol Group Acceptor) / metabolism Platelet-Derived Growth Factor / pharmacology Protein-Serine-Threonine Kinases* Proto-Oncogene Proteins / genetics, metabolism* Proto-Oncogene Proteins c-akt Rats Rats, Wistar Transfection |
| Chemical | |
Reg. No./Substance:
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0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/Platelet-Derived Growth Factor; 0/Proto-Oncogene Proteins; 0/Slc2a4 protein, rat; 11061-68-0/Insulin; 78111-17-8/Okadaic Acid; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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