Document Detail

Potential role of NADH oxidoreductase-derived reactive O2 species in calf pulmonary arterial PO2-elicited responses.
MedLine Citation:
PMID:  7491983     Owner:  NLM     Status:  MEDLINE    
Our laboratory has previously reported evidence that tone responses of isolated endothelium-removed calf pulmonary arteries elicited by changes in PO2 appear to be mediated via changes in H2O2 and guanosine 3',5'-cyclic monophosphate, and that the PO2 sensor mechanism is hypothesized to involve changes in superoxide anion (O2-.) production by a microsomal NADH-oxidoreductase, which is the major source of O2-. detected by lucigenin-elicited chemiluminescence (CL) in this tissue. In this study we examined if the flavoprotein-directed inhibitor of O2-. producing NAD(P)H oxidoreductases, diphenyliodonium (DPI), could be employed as an inhibitor of O2-. production by NADH oxidoreductase, which functions as a selective probe for PO2-elicited tone responses in calf pulmonary arterial smooth muscle. It was found that 1 microM DPI inhibited NADH-dependent production of CL in the arterial smooth muscle homogenate by 49% (n = 10). DPI reduced basal CL from endothelium-removed pulmonary arteries by 41% (n = 15). In endothelium-removed pulmonary arteries precontracted with U-46619, the hypoxic contraction of 2.3 +/- 0.5 g was reduced to 0.1 +/- 0.4 g (n = 7) by DPI, and the reoxygenation relaxation of 32.7 +/- 7.5% was decreased to 4.4 +/- 1.4% (n = 7). DPI did not have any significant effect on U-46619- or K(+)-elicited tone generation. DPI also did not alter the relaxation to H2O2 (1 microM-0.1 mM, n = 6), nitric oxide (0.42 nM-420 nM, n = 12), or isoproterenol (1 nM-1 microM, n = 6).(ABSTRACT TRUNCATED AT 250 WORDS)
K M Mohazzab; R P Fayngersh; P M Kaminski; M S Wolin
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  269     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-01-04     Completed Date:  1996-01-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  L637-44     Citation Subset:  IM    
Department of Physiology, New York Medical College, Valhalla 10595, USA.
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MeSH Terms
Biphenyl Compounds / pharmacology
Chemiluminescent Measurements
Flavoproteins / metabolism
Histological Techniques
Muscle, Smooth / metabolism
NAD / metabolism*
Onium Compounds / pharmacology
Oxidoreductases / metabolism*
Oxygen / metabolism*
Partial Pressure
Pulmonary Artery / physiology*
Reactive Oxygen Species / metabolism*
Vasoconstriction / drug effects
Vasodilation / drug effects
Grant Support
Reg. No./Substance:
0/Biphenyl Compounds; 0/Flavoproteins; 0/Onium Compounds; 0/Reactive Oxygen Species; 10182-84-0/diphenyliodonium; 53-84-9/NAD; 7782-44-7/Oxygen; EC 1.-/Oxidoreductases

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