| Potential proteomic biomarkers in assessing liver fibrosis using SELDI-TOF MS. | |
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MedLine Citation:
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PMID: 22505379 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background/aims: The accurate assessment of the severity of liver fibrosis is of paramount importance in determining treatment strategies, response to treatment and prognosis in patients with chronic liver disease. The aim of this study was to investigate potential proteomic biomarkers for assessing stages of hepatic fibrosis. Methods: Serum samples of 83 patients with chronic liver disease (using METAVIR index, 17 F0, 30 F1, 6 F2, 9 F3, and 21 F4 patients) and 29 healthy controls were analyzed using surfaceenhanced laser desorption/ionization time-of- flight mass spectrometry on IMAC30 ProteinChip arrays. Discriminatory peaks between groups were identified using Mann-Whitney U non-parametric test. Comparison of mild (F0, F1) and severe fibrosis (F2, F3, F4) was performed using tree classification (cross-validation) with the Biomarker Patterns Software, version 5.0 (Ciphergen Biosystems, US). Results: No statistically significant discriminatory peak was evident between F0, F1 and F2 fibrosis. More than 30 peaks were found to be discriminatory between patients with cirrhosis (F4) and all other stages of liver fibrosis, including F2 and F3. Six surface-enhanced laser desorption/ionization proteomic features were found to be discriminative for mild (F0, F1) vs. advanced (F2, F3, F4) fibrosis (AUROC ≥0.8, p<0.05, Mann-Whitney test). The decision tree (m/z 4280, 10453 and 6376) yielded a sensitivity of 83.3% (30/36), a specificity of 85.1% (40/47), a positive predictive value of 81.1%, and a negative predictive value of 86.9%, with an AUROC of 0.94. Conclusions: The results of this study revealed discriminatory peaks between the protein profiles of patients with cirrhosis and other stages of liver fibrosis. Potential proteomic biomarkers can be notably determined for discriminating mild and advanced fibrosis using surface-enhanced laser desorption/ionization time-of- flight mass spectrometry. |
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Authors:
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A Mithat Bozdayi; Senem Ceren Karatayli; S Gökçe Alagöz; Dilşa Mizrak; Müyesser Sayki; Muhip Ozkan; Berna Savaş; Esra Erden; Kubilay Cinar; Ramazan Idılman; Cihan Yurdaydin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology Volume: 23 ISSN: 1300-4948 ISO Abbreviation: Turk J Gastroenterol Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-04-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9515841 Medline TA: Turk J Gastroenterol Country: Turkey |
Other Details:
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Languages: eng Pagination: 46-53 Citation Subset: IM |
Affiliation:
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Ankara University, School of Medicine Institute of Hepatology, Ankara, Turkey. mithat.bozdayi@medicine.ankara.edu.tr. |
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