| Potential problems in the use of commercial preparations of radiolabeled cholesterol. | |
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MedLine Citation:
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PMID: 2282104 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Through a series of biological and analytical procedures, we demonstrate that a compound purchased from a commercial supplier as [7-3H]cholesterol was not cholesterol. In mouse peritoneal macrophages, this compound was metabolized differently than other radiolabeled cholesterol preparations and was accumulated in the steryl ester pool. In contrast, Fu5AH rat hepatoma cells did not discriminate this compound from cholesterol. Further analysis of the anomalous [7-3H]cholesterol by TLC after cholesterol oxidase treatment and by HPLC indicated that this radiochemical was less polar than cholesterol standard and other radiolabeled cholesterol preparations tested. Mass spectrometry analysis disclosed that the chemical has a similar fragmentation pattern and the same molecular weight (386) as cholesterol. |
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Authors:
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F H Mahlberg; A Rodriguez-Oquendo; D W Bernard; J M Glick; G H Rothblat |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Atherosclerosis Volume: 84 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1991-03-13 Completed Date: 1991-03-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 95-100 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia 19129. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cholesterol / chemistry, metabolism, standards* Chromatography, High Pressure Liquid Chromatography, Thin Layer Liver Neoplasms, Experimental / metabolism Macrophages / metabolism Tritium* |
| Grant Support | |
ID/Acronym/Agency:
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HL-07443/HL/NHLBI NIH HHS; HL-22633/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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10028-17-8/Tritium; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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