| Potential for pre-implantation determination of human platelet antigen type using DNA amplification: a strategy for prevention of allo-immune thrombocytopenia. | |
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MedLine Citation:
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PMID: 7945902 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To demonstrate that fetal human platelet antigen (HPA1) type can be determined by amplification from picogram quantities of DNA, nested oligonucleotide DNA primers were designed to amplify a portion of the platelet glycoprotein GpIIIa gene which spans the polymorphic HPA site. Specific oligonucleotides were designed to hybridise either to the amplified HPA1a allele or to the HPA1b allele. DNA dilutions were used as a template and HPA1 type, determined by this method from as little as 2 pg of DNA in 6 cases, was compared to direct typing of blood from the same individuals. In each case nested polymerase chain reaction amplified the specific HPA locus from as little as 2 pg of DNA and determination of HPA1 type agreed with typing of blood. |
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Authors:
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P R Bennett; J Vaughan; A Handyside; N M Fisk |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Fetal diagnosis and therapy Volume: 9 ISSN: 1015-3837 ISO Abbreviation: Fetal. Diagn. Ther. Publication Date: 1994 Jul-Aug |
Date Detail:
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Created Date: 1994-12-08 Completed Date: 1994-12-08 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9107463 Medline TA: Fetal Diagn Ther Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 229-32 Citation Subset: IM |
Affiliation:
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Royal Postgraduate Medical School, Institute of Obstetrics and Gynaecology, Queen Charlotte's and Chelsea Hospital, London, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Human Platelet
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genetics* Base Sequence Blood Grouping and Crossmatching DNA / analysis* Embryonic Development* Female Humans Isoantibodies* Molecular Sequence Data Nucleic Acid Hybridization Platelet Membrane Glycoproteins / genetics Polymerase Chain Reaction* Pregnancy Thrombocytopenia / immunology*, prevention & control |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Human Platelet; 0/Isoantibodies; 0/Platelet Membrane Glycoproteins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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