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Potential mechanisms explaining why hydrolysed casein based diets outclass single amino acid based diets in the prevention of Autoimmune Diabetes in Diabetes Prone BB rats.
MedLine Citation:
PMID:  22539454     Owner:  NLM     Status:  Publisher    
BACKGROUND: It remains controversial whether avoidance of dietary diabetogenic triggers, e.g. cow's milk proteins, can prevent T1D in genetically susceptible individuals. Here different extensive casein hydrolysates (HC) and single amino acid (AA) formulations were tested for their effect on mechanisms underlying autoimmune diabetes pathogenesis in DP-BB rats. Intestinal integrity, gut microbiota composition and mucosal immune reactivity were studies to assess whether these formulations have differential effects in autoimmune diabetes prevention. METHODS: DP-BB rats received diets in which the protein fraction was exchanged for the different hydrolysate or AA compositions, starting from weaning until the end of the experiment (d150). diabetes development was monitored and fecal and ileal samples collected. Gut microbiota composition and cytokine/tight junction mRNA expression were measured by qPCR. Cytokine levels of ileum explant cultures were measured by ELISA and intestinal permeability was measured in-vivo by Lactulose-Mannitol (LA/MA) assay. RESULTS: Both HC-diet fed groups revealed remarkable reduction of diabetes incidence with the most pronounced effect in Nutramigen®-fed animals. Interestingly AA-fed rats only showed delayed autoimmune diabetes development. Furthermore both HC-fed groups had improved intestinal barrier function when compared to control chow or AA-fed animals. Interestingly, higher IL-10 levels were measured in ileum tissue explants from Nutramigen®-fed rats. Beneficial gut microbiota changes (increased Lactobacilli and reduced Bacteroides spp levels) were found associated especially with HC-diet interventions. CONCLUSIONS: Casein hydrolysates were found superior to AA-mix in autoimmune diabetes prevention. This suggests the presence of specific peptides that beneficially affect mechanisms that may play a critical role in autoimmune diabetes pathogenesis. Copyright © 2012 John Wiley & Sons, Ltd.
J T J Visser; N A Bos; L F Harthoorn; F Stellaard; S Beijer-Liefers; J Rozing; E A F van Tol
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-27
Journal Detail:
Title:  Diabetes/metabolism research and reviews     Volume:  -     ISSN:  1520-7560     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883450     Medline TA:  Diabetes Metab Res Rev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Dept. of Cell Biology, Section Immunology, University Medical Center Groningen, University of Groningen, the Netherlands.
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