| Potential mechanism in sonodynamic therapy and focused ultrasound induced apoptosis in sarcoma 180 cells in vitro. | |
| | |
MedLine Citation:
|
PMID: 19640555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sonodynamic therapy employs a combination of ultrasound and a sonosensitizer to enhance the cytotoxic effect of ultrasound and promote apoptosis. However, the mechanism underlying the synergistic effect of ultrasound and hematoporphyrin is still unclear. In this study, we investigated mechanism of the induction of apoptosis by sonodynamic therapy in Sarcoma 180 cells. The cell suspension was treated by 1.75-MHz focused continuous ultrasound at an acoustic power (I(SATA)) of 1.4+/-0.07 W/cm(2) for 3 min in the absence or presence of 20 microg/ml hematoporphyrin. The proportion of apoptotic cells was determined by flow cytometry. We then analyzed the reactive oxygen species generation and localization by confocal microscopy. Western blotting and reverse transcriptase-polymerase chain reaction were used to analyze the expression of caspase-8, caspase-9, poly(ADP)-ribose polymerase, and nuclear factor-kappaB. The findings of our study indicate that ultrasound treatment induced the activation of nuclear factor-kappaB as an early stress response. When cells were pretreated with hematoporphyrin, the initial response to the therapy was the formation of (1)O(2) in the mitochondria. Our results primarily demonstrate that the mechanisms of induction of apoptosis by ultrasound and hematoporphyrin-sonodynamic therapies are very different. Our findings can provide a basis for explaining the synergistic effect of ultrasound and hematoporphyrin. |
| | |
Authors:
|
Wei Tang; Quanhong Liu; Xiaobing Wang; Pan Wang; Jing Zhang; Bing Cao |
Related Documents
:
|
17003475 - Alpha-1 antitrypsin inhibits caspase-3 activity, preventing lung endothelial cell apopt... 10193575 - Hydrogen peroxide-induced apoptosis in hl-60 cells requires caspase-3 activation. 8600315 - Lactate stimulation of macrophage-derived angiogenic activity is associated with inhibi... 10772815 - Adenovirus-mediated transfer of caspase-3 with fas ligand induces drastic apoptosis in ... 20181055 - Enhancement of fibrinogen-triggered pro-coagulant activation of monocytes in vitro by m... 21383505 - Caffeic acid phenethyl ester reduces spinal cord injury-evoked locomotor dysfunction. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-02 |
Journal Detail:
|
Title: Ultrasonics Volume: 49 ISSN: 1874-9968 ISO Abbreviation: Ultrasonics Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-11-09 Completed Date: 2009-12-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0050452 Medline TA: Ultrasonics Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 786-93 Citation Subset: IM |
Affiliation:
|
College of Life Sciences, Shaanxi Normal University, Shaanxi, Xi'an 710062, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / radiation effects* Feasibility Studies Sarcoma 180 Ultrasonic Therapy / methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative study of the characteristics and properties of tendinocytes derived from three tendons i...
Next Document: In vivo attenuation and equivalent scatterer size parameters for atherosclerotic carotid plaque: pre...