Document Detail

Potential induction of rat muscle-derived stem cells to neural-like cells by retinoic acid.
MedLine Citation:
PMID:  20740690     Owner:  NLM     Status:  In-Data-Review    
Several recent studies have demonstrated that stem cell differentiation can be generated by derivatives of retinoic acid. In this study we chose retinoic acid (RA) for inducing neural differentiation of rat muscle-derived stem cells (rMDSCs). rMDSCs were pre-induced with 10 ng/ml basic fibroblast growth factor (bFGF) and then treated with 2 μM RA. After stimulation, RA induced rMDSCs to have a neural-like morphology after 1-7 days of in vitro differentiation. In the results of immunocytochemistry, rMDSC treated with RA showed abundant positive cells against the neuronal markers neuronal-specific enolase (NSE) and tubulin-βIII (Tuj1). Also, 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)-positive cells were observed, indicating oligodendrocyte lineage cells. However, positive cells against glial fibrillary acidic protein (GFAP), marker of astrocytes, were not detected. The mRNA profile of these cells included higher expression of NSE compared with those of non-treated cells in real-time PCR. From the data in this work, we suggest that rMDSCs can trans-differentiate into a neural-like phenotype under the RA conditions. Copyright © 2010 John Wiley & Sons, Ltd.
E Sle Kim; Gyeong Hae Kim; Mi Lan Kang; Yun Mi Kang; Kkot Nim Kang; Ki Chul Hwang; Byoung Hyun Min; Jae Ho Kim; Moon Suk Kim
Publication Detail:
Type:  Journal Article     Date:  2010-08-26
Journal Detail:
Title:  Journal of tissue engineering and regenerative medicine     Volume:  5     ISSN:  1932-7005     ISO Abbreviation:  J Tissue Eng Regen Med     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101308490     Medline TA:  J Tissue Eng Regen Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  410-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 John Wiley & Sons, Ltd.
Department of Molecular Science and Technology, Ajou University, Suwon 443-759, Korea.
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