Document Detail

Potential implications for monitoring serum bile acid profiles in circulation with serum proteome for carbon tetrachloride-induced liver injury/regeneration model in mice.
MedLine Citation:
PMID:  20583827     Owner:  NLM     Status:  MEDLINE    
Bile acids have recently emerged as versatile signaling molecules, and their signaling pathway is a promising target for the treatment of metabolic diseases. Here, we developed a highly sensitive and high-throughput quantification method for six taurine- and glycine-conjugated bile acids using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry after solid-phase extraction (SPE-MALDI-TOF MS). In a carbon tetrachloride (CCl4)-induced liver injury/regeneration model in mice, serum bile acid profiles were monitored, and the same samples were separated by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), and protein spots that significantly changed in quantity in a serial time points were identified by MALDI-TOF MS. Serum taurocholic acid (TCA) concentration was significantly elevated earlier than the increase of serum aspartate aminotransferase (AST)/alanine aminotransferase (ALT) activity, a potentially sensitive marker for minimal hepatic damage. Furthermore, TCA peaked at 20 h after treatment when massive serum proteins appeared in circulation. It should be noted that direct MALDI-imaging mass spectrometry (IMS) has succeeded in showing a hepatic lobular distribution change of TCA, predominantly seen in zone 1 area whereas necrotic changes were dominant in zone 3 area. The in-depth analysis of bile acid profiles in circulation with hepatic lobular distribution is a strong basis to understand the serum proteome in CCl4-induced liver injury model.
Takashi Shimada; Tsuyoshi Nakanishi; Atsuhiko Toyama; Satoshi Yamauchi; Atsuhiro Kanzaki; Hideshi Fujiwake; Taka-Aki Sato; Masaya Ikegawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of proteome research     Volume:  9     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-03     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4490-500     Citation Subset:  IM    
Life Science Research Center, Shimadzu Corporation.
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MeSH Terms
Alanine Transaminase / metabolism
Analysis of Variance
Aspartate Aminotransferases / metabolism
Bile Acids and Salts / blood*
Biological Markers / blood
Blood Proteins / analysis*
Carbon Tetrachloride Poisoning / blood*
Drug-Induced Liver Injury / blood*
Electrophoresis, Gel, Two-Dimensional
Glycine / metabolism
Liver Regeneration / physiology*
Mice, Inbred ICR
Proteomics / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Taurine / metabolism
Taurocholic Acid / blood
Reg. No./Substance:
0/Bile Acids and Salts; 0/Biological Markers; 0/Blood Proteins; 107-35-7/Taurine; 56-40-6/Glycine; 81-24-3/Taurocholic Acid; EC Aminotransferases; EC Transaminase

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