| Potential implications for monitoring serum bile acid profiles in circulation with serum proteome for carbon tetrachloride-induced liver injury/regeneration model in mice. | |
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MedLine Citation:
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PMID: 20583827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bile acids have recently emerged as versatile signaling molecules, and their signaling pathway is a promising target for the treatment of metabolic diseases. Here, we developed a highly sensitive and high-throughput quantification method for six taurine- and glycine-conjugated bile acids using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry after solid-phase extraction (SPE-MALDI-TOF MS). In a carbon tetrachloride (CCl4)-induced liver injury/regeneration model in mice, serum bile acid profiles were monitored, and the same samples were separated by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), and protein spots that significantly changed in quantity in a serial time points were identified by MALDI-TOF MS. Serum taurocholic acid (TCA) concentration was significantly elevated earlier than the increase of serum aspartate aminotransferase (AST)/alanine aminotransferase (ALT) activity, a potentially sensitive marker for minimal hepatic damage. Furthermore, TCA peaked at 20 h after treatment when massive serum proteins appeared in circulation. It should be noted that direct MALDI-imaging mass spectrometry (IMS) has succeeded in showing a hepatic lobular distribution change of TCA, predominantly seen in zone 1 area whereas necrotic changes were dominant in zone 3 area. The in-depth analysis of bile acid profiles in circulation with hepatic lobular distribution is a strong basis to understand the serum proteome in CCl4-induced liver injury model. |
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Authors:
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Takashi Shimada; Tsuyoshi Nakanishi; Atsuhiko Toyama; Satoshi Yamauchi; Atsuhiro Kanzaki; Hideshi Fujiwake; Taka-Aki Sato; Masaya Ikegawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of proteome research Volume: 9 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-03 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 4490-500 Citation Subset: IM |
Affiliation:
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Life Science Research Center, Shimadzu Corporation. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine Transaminase
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metabolism Analysis of Variance Animals Aspartate Aminotransferases / metabolism Bile Acids and Salts / blood* Biological Markers / blood Blood Proteins / analysis* Carbon Tetrachloride Poisoning / blood* Drug-Induced Liver Injury / blood* Electrophoresis, Gel, Two-Dimensional Glycine / metabolism Histocytochemistry Liver Regeneration / physiology* Male Mice Mice, Inbred ICR Proteomics / methods* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Taurine / metabolism Taurocholic Acid / blood |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Biological Markers; 0/Blood Proteins; 107-35-7/Taurine; 56-40-6/Glycine; 81-24-3/Taurocholic Acid; EC 2.6.1.1/Aspartate Aminotransferases; EC 2.6.1.2/Alanine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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