| Potential implication of new torque teno mini viruses in parapneumonic empyema in children. | |
| | |
MedLine Citation:
|
PMID: 23060626 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
An unexplained increasing incidence of parapneumonic empyema (PPE) in pneumonia cases has been reported in recent years. The present study investigated the genetic and biological specifications of new isolates of Torque Teno Mini Virus (TTMV) detected in pleural effusion (PE) samples from children hospitalised for severe pneumonia with PPE.A pathogen discovery protocol was applied in undiagnosed PE samples and led us to the identification of 3 new isolates of TTMV (TTMV-LY). Isolated TTMV-LY genomes were transfected into A549 and HEK293T cells and viral replication was assessed by qPCR and full-length genome amplification. A549 cells were further infected with released TTMV-LY virions and the induced-innate immune response was measured by multiplex immunoassays.Genetic analyses of the 3 TTMV-LY genomes revealed a classic genomic organisation but a weak identity (<64%) with known sequences. We demonstrated here the in vitro replication of TTMV-LY in alveolar epithelial cells and the effective release of infectious viral particles. We also showed a selective production of inflammatory mediators in response to TTMV infection.This study reports the description of replicative TTMV-LY isolated from parapneumonic effusions of children hospitalised with PPE, suggesting the potential role of the virus in the pathogenesis of pneumonia. |
| | |
Authors:
|
Johanna Galmès; Yongjun Li; Alain Rajoharison; Lili Ren; Sandra Dollet; Nathalie Richard; Guy Vernet; Etienne Javouhey; Jianwei Wang; Jean-Noël Telles; Gláucia Paranhos-Baccalà |
Related Documents
:
|
23094116 - Hantavirus pulmonary syndrome in santa cruz, bolivia: outbreak investigation and antibo... 23475426 - A model for the spatial transmission of dengue with daily movement between villages and... 2167606 - Coxsackie b antigen in the central nervous system of a patient with fatal acute encepha... 22969426 - The escrt machinery is recruited by the viral bfrf1 protein to the nucleus-associated m... 12019106 - Emergence of resistant human immunodeficiency virus type 1 in patients receiving fusion... 1917486 - Recognition of microclimate zones through radon mapping, lechuguilla cave, carlsbad cav... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-11 |
Journal Detail:
|
Title: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology Volume: - ISSN: 1399-3003 ISO Abbreviation: Eur. Respir. J. Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8803460 Medline TA: Eur Respir J Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Fondation Mérieux, Lyon, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intrabronchial activated protein C enhances lipopolysaccharide-induced pulmonary responses.
Next Document: Identification of asthma clusters in two independent Korean adult asthma cohorts.