Document Detail


Potential of human saliva for nuclear magnetic resonance-based metabolomics and for health-related biomarker identification.
MedLine Citation:
PMID:  19780580     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, the ability of (1)H nuclear magnetic resonance (NMR) for metabolic profiling of human saliva samples was investigated. High-resolution (1)H NMR spectra were obtained, and signals were assigned to various metabolites mainly representing small organic acids and amino acids. In addition, the use of human saliva for metabolomic studies was evaluated, and multivariate data analysis revealed that the 92 morning and night samples from 46 subjects could be discriminated with a predictability of 85%. The diurnal effect on the salivary metabolite profile were ascribed to changes in intensities of several metabolites including trimethylamine oxide (TMAO), choline, propionate, alanine, methanol, and N-acetyl groups. No effects of gender and body mass index (BMI) on the salivary metabolite profile were detected. The relationships between the salivary metabolome and glycated hemoglobin, systolic and diastolic blood pressure were investigated; however, no significant correlations could be established.
Authors:
Hanne Christine Bertram; Nina Eggers; Nanna Eller
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Technical Report    
Journal Detail:
Title:  Analytical chemistry     Volume:  81     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-30     Completed Date:  2010-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9188-93     Citation Subset:  IM    
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, Aarhus University, P.O. Box 102, DK-5792 Arslev, Denmark. HanneC.Bertram@agrsci.dk
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / analysis*
Humans
Metabolomics / methods*
Nuclear Magnetic Resonance, Biomolecular / methods*
Principal Component Analysis
Saliva / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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