Document Detail


Potential of genes and gene products from Trichoderma sp. and Gliocladium sp. for the development of biological pesticides.
MedLine Citation:
PMID:  7866877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fungal cell wall degrading enzymes produced by the biocontrol fungi Trichoderma harzianum and Gliocladium virens are strong inhibitors of spore germination and hyphal elongation of a number of phytopathogenic fungi. The purified enzymes include chitinolytic enzymes with different modes of action or different substrate specificity and glucanolytic enzymes with exo-activity. A variety of synergistic interactions were found when different enzymes were combined or associated with biotic or abiotic antifungal agents. The levels of inhibition obtained by using enzyme combinations were, in some cases, comparable with commercial fungicides. Moreover, the antifungal interaction between enzymes and common fungicides allowed the reduction of the chemical doses up to 200-fold. Chitinolytic and glucanolytic enzymes from T. harzianum were able to improve substantially the antifungal ability of a biocontrol strain of Enterobacter cloacae. DNA fragments containing genes encoding for different chitinolytic enzymes were isolated from a cDNA library of T. harzianum and cloned for mechanistic studies and biocontrol purposes. Our results provide additional information on the role of lytic enzymes in processes of biocontrol and strongly suggest the use of lytic enzymes and their genes for biological control of plant diseases.
Authors:
M Lorito; C K Hayes; A Zoina; F Scala; G Del Sorbo; S L Woo; G E Harman
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Molecular biotechnology     Volume:  2     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-03-27     Completed Date:  1995-03-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  209-17     Citation Subset:  IM    
Affiliation:
Istituto di Patologia Vegetale, Università degli Studi di Napoli Federico II, Portici, Italy.
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MeSH Terms
Descriptor/Qualifier:
Acetylglucosaminidase / genetics,  isolation & purification,  pharmacology
Cell Wall / drug effects
Chitinase / genetics,  isolation & purification,  pharmacology
Cloning, Molecular
DNA, Complementary / genetics
DNA, Fungal / genetics
Drug Design
Drug Synergism
Enterobacter cloacae / physiology
Fungal Proteins / genetics,  isolation & purification,  pharmacology*
Fungi / drug effects,  physiology
Fungicides, Industrial* / pharmacology
Genes, Fungal*
Glucosidases / genetics,  isolation & purification,  pharmacology
Hexosaminidases / genetics,  isolation & purification,  pharmacology
Mitosporic Fungi / enzymology,  genetics*
Pest Control, Biological*
Spores, Fungal / drug effects
Trichoderma / enzymology,  genetics*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Fungal; 0/Fungal Proteins; 0/Fungicides, Industrial; EC 3.2.1.-/1,4-beta-chitobiosidase; EC 3.2.1.-/Glucosidases; EC 3.2.1.-/Hexosaminidases; EC 3.2.1.14/Chitinase; EC 3.2.1.52/Acetylglucosaminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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