| Potential dissemination of Bacillus anthracis utilizing human lung epithelial cells. | |
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MedLine Citation:
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PMID: 18067609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dissemination of Bacillus anthracis spores from the lung is a critical early event in the establishment of inhalational anthrax. We recently reported that B. anthracis could adhere to and be internalized by cultured intestinal epithelial and fibroblast cells. Here, using gentamicin protection assays and/or electron microscopy, we found that Sterne strain 7702 spores were able to adhere to and subsequently be internalized by polarized A549 cells and primary human small airway epithelial cells. We showed for the first time that internalized spores were able to survive and that spores could translocate across an A549 cell barrier from the apical side to the basolateral side without disrupting the barrier integrity, suggesting a transcellular route. In addition, dormant spores of fully virulent Ames and UT500 strains were able to adhere to A549 cells at a frequency similar to that of 7702, whereas the capsule in germinated Ames and UT500 spores prevented adherence. Fluorescence microscopy also revealed that dormant Ames spores were internalized at a frequency similar to that of 7702. These findings highlight the possibility of a novel route of dissemination in which B. anthracis utilizes epithelial cells of the lung. The implications of these results to B. anthracis pathogenesis are discussed. |
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Authors:
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Brooke H Russell; Ranga Vasan; Douglas R Keene; Theresa M Koehler; Yi Xu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-12-06 |
Journal Detail:
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Title: Cellular microbiology Volume: 10 ISSN: 1462-5822 ISO Abbreviation: Cell. Microbiol. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-14 Completed Date: 2008-08-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883691 Medline TA: Cell Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 945-57 Citation Subset: IM |
Affiliation:
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Center for Extracellular Matrix Biology, Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, TX 77030, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus anthracis
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physiology*,
ultrastructure Cell Line Epithelial Cells / cytology, microbiology* Humans Lung / cytology Microscopy, Electron, Scanning Microscopy, Electron, Transmission Microscopy, Fluorescence Spores, Bacterial / physiology, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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AI061555/AI/NIAID NIH HHS; U54 AI057156/AI/NIAID NIH HHS |
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