Document Detail


Potential dissemination of Bacillus anthracis utilizing human lung epithelial cells.
MedLine Citation:
PMID:  18067609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dissemination of Bacillus anthracis spores from the lung is a critical early event in the establishment of inhalational anthrax. We recently reported that B. anthracis could adhere to and be internalized by cultured intestinal epithelial and fibroblast cells. Here, using gentamicin protection assays and/or electron microscopy, we found that Sterne strain 7702 spores were able to adhere to and subsequently be internalized by polarized A549 cells and primary human small airway epithelial cells. We showed for the first time that internalized spores were able to survive and that spores could translocate across an A549 cell barrier from the apical side to the basolateral side without disrupting the barrier integrity, suggesting a transcellular route. In addition, dormant spores of fully virulent Ames and UT500 strains were able to adhere to A549 cells at a frequency similar to that of 7702, whereas the capsule in germinated Ames and UT500 spores prevented adherence. Fluorescence microscopy also revealed that dormant Ames spores were internalized at a frequency similar to that of 7702. These findings highlight the possibility of a novel route of dissemination in which B. anthracis utilizes epithelial cells of the lung. The implications of these results to B. anthracis pathogenesis are discussed.
Authors:
Brooke H Russell; Ranga Vasan; Douglas R Keene; Theresa M Koehler; Yi Xu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-12-06
Journal Detail:
Title:  Cellular microbiology     Volume:  10     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-14     Completed Date:  2008-08-07     Revised Date:  2014-05-29    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  945-57     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacillus anthracis / physiology*,  ultrastructure
Cell Line
Epithelial Cells / cytology,  microbiology*
Humans
Lung / cytology
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Spores, Bacterial / physiology,  ultrastructure
Grant Support
ID/Acronym/Agency:
AI061555/AI/NIAID NIH HHS; R21 AI061555/AI/NIAID NIH HHS; U54 AI057156/AI/NIAID NIH HHS; U54 AI057156/AI/NIAID NIH HHS

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