Document Detail

Potential diagnostic value of methylation profile in pleural fluid and serum from cancer patients with pleural effusion.
MedLine Citation:
PMID:  16983705     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The objective of this study was to investigate the diagnostic value of methylation profiles for discrimination between malignant and benign pleural effusions. A secondary objective was to examine the concordance of methylation in samples of serum and pleural fluid. METHODS: The authors used methylation-specific polymerase chain reaction (MSP) analysis to examine the promoter methylation status of 4 genes in patients with pleural effusion: death-associated protein kinase (DAPK), Ras association domain family 1A (RASSF1A), retinoic acid receptor beta (RARbeta), and p16/INK4a. Pleural effusions were collected from 87 patients who had their diagnoses confirmed on cytologic and/or histologic examinations and clinical evolution. Pleural effusions were classified as malignant (n = 53 patients) or benign (n = 34 patients). RESULTS: Methylation was detected in serum from 45.3% of patients with malignant pleural effusions and from 0% of patients with benign pleural effusions, and it was detected in pleural fluid samples from 58.5% of patients with malignant pleural effusions and from 0% of patients with benign pleural effusions (P = .001). The sensitivity of MSP was greater than that of cytologic examination alone (39.1%; P = .001). When MSP was used together with cytologic examination, sensitivity increased to 69.8% (P = .001). CONCLUSIONS: Cell-free methylated DNA in pleural fluid can be detected in patients with neoplastic malignancy in a single extraction by thoracocentesis. Adequate management of the extracted pleural fluid can provide a rapid and reliable diagnosis in patients with pleural effusions who have suspected malignancy. MSP, used together with cytologic examination, may obviate the need for other invasive diagnostic tests.
Susana Benlloch; Jose Marcelo Galbis-Caravajal; Concepcion Martín; Jose Sanchez-Paya; Jose Manuel Rodríguez-Paniagua; Santiago Romero; Juan Jose Mafe; Bartomeu Massutí
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer     Volume:  107     ISSN:  0008-543X     ISO Abbreviation:  Cancer     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2006-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374236     Medline TA:  Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1859-65     Citation Subset:  AIM; IM    
Copyright Information:
2006 American Cancer Society
Research Unit, Alicante University General Hospital, Alicante, Spain.
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MeSH Terms
Aged, 80 and over
DNA Methylation*
Middle Aged
Molecular Diagnostic Techniques
Neoplasms / blood,  diagnosis*,  genetics,  metabolism
Pleural Effusion / etiology,  genetics*,  metabolism
Pleural Effusion, Malignant / diagnosis*,  genetics,  metabolism
Predictive Value of Tests
Sensitivity and Specificity

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