| Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation. | |
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MedLine Citation:
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PMID: 19592527 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli, including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative. In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly(12) to Tyr that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing its potential use in food preservation. |
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Authors:
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María Fernanda Pomares; Raúl A Salomón; Olga Pavlova; Konstantin Severinov; Ricardo Farías; Paula A Vincent |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-07-10 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 75 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-28 Completed Date: 2009-10-22 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 5734-8 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Tucumán), 4000 San Miguel de Tucumán, Tucumán, Argentina. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Substitution
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genetics* Bacteriocins / genetics, metabolism*, pharmacology Chymotrypsin / metabolism* Colony Count, Microbial Escherichia coli O157 / drug effects Food Preservation / methods Food Preservatives / metabolism*, pharmacology Microbial Sensitivity Tests Microbial Viability Mutagenesis, Site-Directed Salmonella enterica / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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GM64530/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacteriocins; 0/Food Preservatives; 1403-96-9/microcin; EC 3.4.21.1/Chymotrypsin |
| Comments/Corrections | |
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