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Potential advantage of constant pressure versus constant flow gradient chromatography for the analysis of small molecules.
MedLine Citation:
PMID:  23040976     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A recent model designed to predict the variation of the flow rate with time in constant pressure (cP) gradient chromatography was validated from an experimental viewpoint for non-retained gradients (methanol-water), incompressible eluent (P<250bar), and in absence of pressure effects on the analyte retention pattern (small molecules). Experimental data confirmed that cP and constant flow (cF) gradients are strictly equivalent if the analysis time is kept constant. The same model was also used to predict the gradient kinetic performance of cP versus cF gradients when the constraint was the maximum inlet pressure at which the column and/or the HPLC system can safely be run. For linear volume gradients of methanol in water (5-95% in volume) and a maximum pressure of 250bar, the same peak capacity as that in cF mode is predicted in cP mode. Also, a reduction of the analysis time by 17.3% was expected. These theoretical results were confirmed by separating a real mixture of about twenty small molecules on either one or two 4.6mm×150mm columns packed with 3.5μm Bridge Ethylene Hybrid (BEH) C(18) particles and run at flow rates smaller than 0.8mL/min and at a maximum inlet pressure of 250bar. The experimental gain in analysis time was 17.6% (1 column) and 20.1% (2 columns in series) for a virtually insignificant loss of peak capacity (-4%).
Authors:
Fabrice Gritti; Joseph J Stankovich; Georges Guiochon
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-11
Journal Detail:
Title:  Journal of chromatography. A     Volume:  -     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
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