| Potential of the Tannerella forsythia S-layer to delay the immune response. | |
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MedLine Citation:
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PMID: 20929722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The periodontal pathogen Tannerella forsythia possesses a glycosylated S-layer as an outermost cell decoration. While the S-layer provides a selection advantage to the bacterium in the natural habitat, its virulence potential remains to be investigated. In the present study, the immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type T. forsythia and an S-layer-deficient mutant were investigated. The mRNA expression levels of the pro-inflammatory mediators IL-1β, TNF-α, and IL-8 were analyzed by qPCR, and the production of the corresponding cytokines was investigated by ELISA. The S-layer-deficient T. forsythia mutant induced significantly higher levels of pro-inflammatory mediators compared with wild-type T. forsythia, especially at the early phase of response. Analysis of these data suggests that the S-layer of T. forsythia is an important virulence factor that attenuates the host immune response to this pathogen by evading the bacterium's recognition by the innate immune system. Abbreviations: DMSO, dimethylsulfoxide; FBS, fetal bovine serum; GAPDH, glycerinaldehyde-3-phosphate-dehydrogenase; HGFs, human gingival fibroblasts; LPS, lipopolysaccharide; MEM, minimal essential medium; MTT, 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide; OD, optical density; PBS, phosphate-buffered saline; qPCR, quantitative polymerase chain-reaction; SD, standard deviation; Tannerella forsythia ATCC 43037, Tf wt; Tannerella forsythia ATCC 43037 S-layer mutant, Tf ΔtfsAB. |
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Authors:
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G Sekot; G Posch; P Messner; M Matejka; X Rausch-Fan; O Andrukhov; C Schäffer |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-06 |
Journal Detail:
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Title: Journal of dental research Volume: 90 ISSN: 1544-0591 ISO Abbreviation: J. Dent. Res. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-23 Completed Date: 2011-02-04 Revised Date: 2011-09-15 |
Medline Journal Info:
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Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: United States |
Other Details:
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Languages: eng Pagination: 109-14 Citation Subset: D; IM |
Affiliation:
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Department of NanoBiotechnology, Vienna Institute of Bio Technology, Universität für Bodenkultur Wien, Muthgasse 11, A-1190 Vienna, Austria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Outer Membrane Proteins
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genetics,
immunology* Bacteroides / genetics, immunology*, pathogenicity Cell Line Cell Survival / immunology Cells, Cultured Fibroblasts / immunology Gingiva / cytology, immunology Humans Immune Evasion / immunology Immunity, Cellular / immunology* Immunity, Innate / immunology Inflammation Mediators / immunology Interleukin-1beta / genetics, immunology Interleukin-8 / genetics, immunology Macrophages / immunology Membrane Glycoproteins / genetics, immunology* Microscopy, Electron, Transmission Mutation / genetics RNA, Messenger / analysis Tumor Necrosis Factor-alpha / genetics, immunology Virulence / immunology |
| Grant Support | |
ID/Acronym/Agency:
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P 20605-B12//Austrian Science Fund FWF |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Inflammation Mediators; 0/Interleukin-1beta; 0/Interleukin-8; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/S-layer proteins; 0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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