| Potential of magnetic resonance spectroscopy in assessing the effect of fatty acids on inflammatory bowel disease in an animal model. | |
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MedLine Citation:
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PMID: 20721632 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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People with inflammatory bowel disease (IBD) are at risk for developing colorectal cancer, and this risk increases at a rate of 1% per year after 8-10 years of having the disease. Saturated and omega-6 polyunsaturated fatty acids (PUFAs) have been implicated in its causation. Conversely, omega-3 PUFAs may have the potential to confer therapeutic benefit. Since proton magnetic resonance spectroscopy ((1)H MRS) combined with pattern recognition methods could be a valuable adjunct to histology, the objective of this study was to analyze the potential of (1)H MRS in assessing the effect of dietary fatty acids on colonic inflammation. Forty male Sprague-Dawley rats were administered one of the following dietary regimens for 2 weeks: low-fat corn oil (omega-6), high-fat corn oil (omega-6), high-fat flaxseed oil (omega-3) or high-fat beef tallow (saturated fatty acids). Half of the animals were fed 2% carrageenan to induce colonic inflammation similar to IBD. (1)H MRS and histology were performed on ex vivo colonic samples, and the (1)H MR spectra were analyzed using a statistical classification strategy (SCS). The histological and/or MRS studies revealed that different dietary fatty acids modulate colonic inflammation differently, with high-fat corn oil being the most inflammatory and high-fat flaxseed oil the least inflammatory. (1)H MRS is capable of identifying the biochemical changes in the colonic tissue as a result of inflammation, and when combined with SCS, this technique accurately differentiated the inflamed colonic mucosa based on the severity of the inflammation. This indicates that MRS could serve as a valuable adjunct to histology in accurately assessing colonic inflammation. Our data also suggest that both the type and the amount of fatty acids in the diet are critical in modulating IBD. |
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Authors:
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Sonal Varma; Michael N A Eskin; Ranjana Bird; Brion Dolenko; Jayadev Raju; Omkar B Ijare; Tedros Bezabeh |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-19 |
Journal Detail:
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Title: Lipids Volume: 45 ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-02 Completed Date: 2010-12-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: Germany |
Other Details:
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Languages: eng Pagination: 843-54 Citation Subset: IM |
Affiliation:
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National Research Council Institute for Biodiagnostics, 435 Ellice Ave., Winnipeg, MB, R3B 1Y6, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Colitis / metabolism*, pathology Colon / drug effects, metabolism, pathology Corn Oil / administration & dosage, pharmacology Dietary Fats, Unsaturated / administration & dosage, pharmacology* Fatty Acids, Omega-3 / administration & dosage, pharmacology Fatty Acids, Omega-6 / administration & dosage, pharmacology Inflammatory Bowel Diseases / metabolism*, pathology Magnetic Resonance Spectroscopy / methods* Male Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats, Unsaturated; 0/Fatty Acids, Omega-3; 0/Fatty Acids, Omega-6; 8001-30-7/Corn Oil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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