| Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells. | |
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MedLine Citation:
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PMID: 16563722 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers [cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA] on human colon cancer cell lines (Caco-2, HT-29 and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 microM, each isomer, even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time-dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+/-2.7% for t9,t11-CLA, 35.9+/-0.3% for c9,t11-CLA and 46.3+/-0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was dose- and time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA. t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in association with apoptosis, whereas Bax levels remained unchanged. These results suggest that decreased expression of Bcl-2 by t9t11-CLA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death, apoptosis. |
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Authors:
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Fumiaki Beppu; Masashi Hosokawa; Leo Tanaka; Hiroyuki Kohno; Takuji Tanaka; Kazuo Miyashita |
Publication Detail:
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Type: Journal Article Date: 2006-03-24 |
Journal Detail:
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Title: The Journal of nutritional biochemistry Volume: 17 ISSN: 0955-2863 ISO Abbreviation: J. Nutr. Biochem. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-20 Completed Date: 2007-01-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010081 Medline TA: J Nutr Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 830-6 Citation Subset: IM |
Affiliation:
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Laboratory of Biofunctional Material Chemistry, Division of Marine Bioscience, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Apoptosis / drug effects, physiology Caco-2 Cells Cell Death / drug effects Cell Survival / drug effects Colonic Neoplasms / drug therapy*, pathology Drug Screening Assays, Antitumor Humans Linoleic Acids, Conjugated / pharmacology* Proto-Oncogene Proteins c-bcl-2 / drug effects, metabolism Tumor Cells, Cultured bcl-2-Associated X Protein / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/BAX protein, human; 0/Linoleic Acids, Conjugated; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 1839-11-8/9,11-linoleic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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