Document Detail

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells.
MedLine Citation:
PMID:  16563722     Owner:  NLM     Status:  MEDLINE    
This study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers [cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA] on human colon cancer cell lines (Caco-2, HT-29 and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 microM, each isomer, even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time-dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+/-2.7% for t9,t11-CLA, 35.9+/-0.3% for c9,t11-CLA and 46.3+/-0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was dose- and time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA. t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in association with apoptosis, whereas Bax levels remained unchanged. These results suggest that decreased expression of Bcl-2 by t9t11-CLA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death, apoptosis.
Fumiaki Beppu; Masashi Hosokawa; Leo Tanaka; Hiroyuki Kohno; Takuji Tanaka; Kazuo Miyashita
Publication Detail:
Type:  Journal Article     Date:  2006-03-24
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  17     ISSN:  0955-2863     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-20     Completed Date:  2007-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  830-6     Citation Subset:  IM    
Laboratory of Biofunctional Material Chemistry, Division of Marine Bioscience, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects,  physiology
Caco-2 Cells
Cell Death / drug effects
Cell Survival / drug effects
Colonic Neoplasms / drug therapy*,  pathology
Drug Screening Assays, Antitumor
Linoleic Acids, Conjugated / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / drug effects,  metabolism
Tumor Cells, Cultured
bcl-2-Associated X Protein / drug effects,  metabolism
Reg. No./Substance:
0/Antineoplastic Agents; 0/BAX protein, human; 0/Linoleic Acids, Conjugated; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; 1839-11-8/9,11-linoleic acid

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