Document Detail

Potato tuber proteins efficiently inhibit human faecal proteolytic activity: implications for treatment of peri-anal dermatitis.
MedLine Citation:
PMID:  15086363     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Frequent diarrhoea after intestinal resections and faecal incontinence in healthy infants may lead to perianal injury. A causative agent may be a high concentration of pancreatic proteases in faeces. The aim of the present study was to assess whether protease inhibitors are applicable for treating and preventing peri-anal dermatitis by inhibiting the initial cause of the inflammation, the faecal proteases. DESIGN: Proteolytic activity was estimated in faeces of subjects frequently suffering from peri-anal dermatitis: patients with intestinal resections and healthy infants. The development of perianal dermatitis was studied after the construction of a reservoir with ileoanal anastomosis. The inhibitory effect of crude and partly purified potato juice on proteolytic activity of faecal output from patients with intestinal resections and healthy infants was investigated in vitro and in vivo (skin tests). RESULTS: Faecal protease activity in faeces from patients with intestinal resections and healthy infants was found to be significantly higher than in healthy adults. After the construction of an ileum reservoir, 46 of 48 patients developed a protease-related peri-anal dermatitis. The partly purified protein fraction from potatoes inhibited the larger part of faecal proteases in vitro and completely prevented skin irritation by pancreatic proteases dissolved in sterilized faecal fluid, in a 24-h skin test, on the back of healthy human volunteers. CONCLUSIONS: Potato proteins contain protease inhibitors, which suppress almost the complete proteolytic activity in faeces. Topical application of potato protease inhibitors might be a novel approach in preventing protease-induced peri-anal dermatitis, and therapeutic studies are needed to confirm our results.
J G H Ruseler-van Embden; L M C van Lieshout; S A Smits; I van Kessel; J D Laman
Related Documents :
12804473 - Early skin-to-skin contact for mothers and their healthy newborn infants.
16231563 - Methicillin-resistant staphylococcus aureus bacteremia in neonatal intensive care units...
21982303 - Intravenous fat emulsions reduction for patients with parenteral nutrition-associated l...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of clinical investigation     Volume:  34     ISSN:  0014-2972     ISO Abbreviation:  Eur. J. Clin. Invest.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-16     Completed Date:  2004-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0245331     Medline TA:  Eur J Clin Invest     Country:  England    
Other Details:
Languages:  eng     Pagination:  303-11     Citation Subset:  IM    
University Medical Center Rotterdam, Rotterdam, the Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenomatous Polyposis Coli / surgery
Administration, Topical
Anal Canal / surgery
Anastomosis, Surgical / methods
Child, Preschool
Colitis, Ulcerative / surgery
Crohn Disease / surgery
Dermatitis / drug therapy*
Feces / enzymology*
Hypersensitivity / etiology
Ileum / surgery
Middle Aged
Plant Proteins / administration & dosage,  adverse effects,  therapeutic use*
Plant Tubers / metabolism*
Protease Inhibitors / therapeutic use*
Skin Tests
Solanum tuberosum / metabolism*
Reg. No./Substance:
0/Plant Proteins; 0/Protease Inhibitors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Evaluation of effects of rofecoxib on platelet function in an in vitro model of thrombosis with circ...
Next Document:  Are there characteristics of infectious diseases that raise special ethical issues?