| Potato cultivar genome analysis. | |
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MedLine Citation:
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PMID: 19301764 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Due to the yearly increase in the numbers of new potato varieties obtaining Plant Breeders' Rights the reliable maintenance of large culture collections of reference varieties for DUS testing is becoming more and more difficult, as accidental mix ups might occur. Efficient identification methods and databases can act as an aid to overcome this problem. Identification of cultivars by morphological characteristics is a highly skilled and time-consuming task, and for these reasons a rapid and robust method for variety differentiation has become extremely desirable. By use of a set of nine microsatellite (SSR) markers we can differentiate over 1,000 cultivars, including the majority of varieties on the European Union Common Catalogue, but excluding somaclonal variants (e.g. Red King Edward and King Edward) and mutants. The whole identification process from DNA extraction to accurate identification can be carried out in a single day. |
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Authors:
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Alex Reid; Lysbeth Hof; Danny Esselink; Ben Vosman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 508 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-03-23 Completed Date: 2009-04-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 295-308 Citation Subset: IM |
Affiliation:
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DMB, Scottish Agricultural Science Agency, Roddinglaw Road, Edinburgh, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Genetic Variation Genome, Plant / genetics Laboratory Techniques and Procedures* Microsatellite Repeats / genetics* Solanum tuberosum / classification, genetics* Species Specificity |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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