Document Detail


Potassium uptake system Trk2 is crucial for yeast cell viability during anhydrobiosis.
MedLine Citation:
PMID:  24267958     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Yeasts grow at very different potassium concentrations adapting their intracellular cation levels to changes in the external environment. Potassium homeostasis is maintained with the help of several transporters mediating the uptake and efflux of potassium with various affinities and mechanisms. In the model yeast S. cerevisiae, two uptake systems, Trk1 and Trk2, are responsible for the accumulation of a relatively high intracellular potassium content (200 - 300 mM), and the efflux of surplus potassium is mediated by the Tok1 channel and active exporters Ena ATPase and Nha1 cation/proton antiporter. Using a series of deletion mutants, we studied the role of individual potassium transporters in yeast cell resistance to dehydration. The Trk2 transporter (whose role in S. cerevisiae physiology was not clear) is important for cell viability in the stationary phase of growth and moreover, it plays a crucial role in the yeast survival of dehydration/rehydration treatments. Mutants lacking the TRK2 gene accumulated significantly lower amounts of potassium ions in the stationary culture growth phase, and these lower amounts correlated with decreased resistance to dehydration/rehydration stress. Our results showed Trk2 to be the major potassium uptake system in stationary cells and potassium content to be a crucial parameter for desiccation survival. This article is protected by copyright. All rights reserved.
Authors:
Diana Borovikova; Pavla Herynkova; Alexander Rapoport; Hana Sychrova
Related Documents :
9858128 - Human red cells from prenatal hemopoiesis. sodium/lithium exchange symmetry.
1314518 - Downregulation of sodium channels during anoxia: a putative survival strategy of turtle...
2998188 - Bartter's syndrome: a unifying hypothesis.
6264798 - Transcellular transport of calcium and inorganic phosphate in the small intestinal epit...
9174358 - Alpha 2a-adrenergic receptor stimulated calcium release is transduced by gi-associated ...
9175888 - Anti-epileptogenic and anticonvulsant activity of l-2-amino-4-phosphonobutyrate, a pres...
Publication Detail:
Type:  LETTER     Date:  2013-11-23
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-11-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
Affiliation:
Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Riga, Latvia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Diagnostic utility of BRAFV600E mutation testing in thyroid nodules in elderly patients.
Next Document:  The effect of contouring variability on dosimetric parameters for brain metastases treated with ster...