Document Detail

Potassium transport and the relationship between intracellular potassium concentration and amino acid uptake by cells of a marine pseudomonad.
MedLine Citation:
PMID:  4455685     Owner:  NLM     Status:  MEDLINE    
Transport of K(+) by K(+)-depleted cells of marine pseudomonad B-16 (ATCC 19855) exhibited saturation kinetics. Rb(+) inhibited both K(+) transport and the K(+)-dependent transport of alpha-aminoisobutyric acid (AIB) into K(+)-depleted cells of the organism in proportion to the concentration of Rb(+) in the suspending medium. Inhibition of the K(+)-dependent uptake of AIB into K(+)-depleted cells by Rb(+) could be overcome by increasing the concentration of K(+) in the medium. When AIB and K(+) were added simultaneously to a suspension of K(+)-depleted cells, the uptake of K(+) occurred immediately and rapidly, whereas the accumulation of AIB occurred only after a lag. The initial uptake rate of AIB was directly proportional to the intracellular K(+) concentration. The intracellular concentration of K(+) and AIB at their steady-state levels increased to a maximum as the Na(+) concentration in the suspending medium was increased. At Na(+) concentrations between 0.2 and 0.3 M, the molar ratio of K(+) to AIB at their intracellular steady-state concentrations was constant at 1.6. At external Na(+) concentrations less than 0.2 M, the cells maintained a relatively higher K(+) intracellular steady-state level than AIB.
J Thompson; R A MacLeod
Related Documents :
3622635 - Genetic demonstration that the mutationally expressed nucleobase transporter of mouse s...
17520485 - Differential regulation of mouse equilibrative nucleoside transporter 1 (ment1) splice ...
19748575 - Inhibition of nucleoside transport and synergistic potentiation of methotrexate cytotox...
22592295 - Galvestine-1, a novel chemical probe for the study of the glycerolipid homeostasis syst...
3069225 - Fate of intraocular chromaffin cell suspensions: role of initial nerve growth factor su...
10461355 - Properties of isolated gastric enterochromaffin-like cells.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  120     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 Nov 
Date Detail:
Created Date:  1975-06-21     Completed Date:  1975-06-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  598-603     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / metabolism*
Aminobutyric Acids / analogs & derivatives,  metabolism
Biological Transport, Active
Carbon Isotopes
Culture Media
Marine Biology
Potassium / antagonists & inhibitors,  metabolism*
Potassium Isotopes
Pseudomonas / cytology*,  growth & development,  metabolism
Rubidium / pharmacology
Sodium / pharmacology
Reg. No./Substance:
0/Amino Acids; 0/Aminobutyric Acids; 0/Carbon Isotopes; 0/Culture Media; 0/Potassium Isotopes; 0/Radioisotopes; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Unidirectional growth and branch formation of a morphological mutant, Agrobacterium tumefaciens.
Next Document:  Regulation of bacterial cell walls: turnover of cell wall in Staphylococcus aureus.