Document Detail


Potassium transport and the relationship between intracellular potassium concentration and amino acid uptake by cells of a marine pseudomonad.
MedLine Citation:
PMID:  4455685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transport of K(+) by K(+)-depleted cells of marine pseudomonad B-16 (ATCC 19855) exhibited saturation kinetics. Rb(+) inhibited both K(+) transport and the K(+)-dependent transport of alpha-aminoisobutyric acid (AIB) into K(+)-depleted cells of the organism in proportion to the concentration of Rb(+) in the suspending medium. Inhibition of the K(+)-dependent uptake of AIB into K(+)-depleted cells by Rb(+) could be overcome by increasing the concentration of K(+) in the medium. When AIB and K(+) were added simultaneously to a suspension of K(+)-depleted cells, the uptake of K(+) occurred immediately and rapidly, whereas the accumulation of AIB occurred only after a lag. The initial uptake rate of AIB was directly proportional to the intracellular K(+) concentration. The intracellular concentration of K(+) and AIB at their steady-state levels increased to a maximum as the Na(+) concentration in the suspending medium was increased. At Na(+) concentrations between 0.2 and 0.3 M, the molar ratio of K(+) to AIB at their intracellular steady-state concentrations was constant at 1.6. At external Na(+) concentrations less than 0.2 M, the cells maintained a relatively higher K(+) intracellular steady-state level than AIB.
Authors:
J Thompson; R A MacLeod
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  120     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1974 Nov 
Date Detail:
Created Date:  1975-06-21     Completed Date:  1975-06-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  598-603     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Aminobutyric Acids / analogs & derivatives,  metabolism
Biological Transport, Active
Carbon Isotopes
Culture Media
Kinetics
Marine Biology
Potassium / antagonists & inhibitors,  metabolism*
Potassium Isotopes
Pseudomonas / cytology*,  growth & development,  metabolism
Radioisotopes
Rubidium / pharmacology
Sodium / pharmacology
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Aminobutyric Acids; 0/Carbon Isotopes; 0/Culture Media; 0/Potassium Isotopes; 0/Radioisotopes; 7440-09-7/Potassium; 7440-17-7/Rubidium; 7440-23-5/Sodium
Comments/Corrections

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