Document Detail

Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line.
MedLine Citation:
PMID:  12620897     Owner:  NLM     Status:  MEDLINE    
Previously, we showed that the peak density of the transient outward K(+) current (I(to)) expressed in GH3 cells was different in the S phase than in other phases of the cell cycle. Using cell synchronization, we show here that I(to) drops precisely at the quiescent (G(0) phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I(to), but rather to a modification in its inactivation kinetics. Molecular determination of K(+) channel subunits showed that I(to) required the expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in I(to) density during the quiescent state was accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3 expression remained unchanged. We further demonstrate that the link between I(to) expression and cell proliferation is not mediated by variations in cell excitability. These results provide new evidence for the cell cycle dependence of I(to) expression, which could be relevant in understanding the mechanisms leading to pituitary adenomas.
Antonny Czarnecki; Luce Dufy-Barbe; Sylvie Huet; Marie-Francoise Odessa; Laurence Bresson-Bepoldin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  284     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-06     Completed Date:  2003-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C1054-64     Citation Subset:  IM    
Laboratoire de Physiologie et Physiopathologie de la Signalisation Cellulaire, CNRS UMR 5543, Université de Bordeaux 2, 33076 Cedex Bordeaux, France.
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MeSH Terms
Cell Cycle
Cell Division / physiology
Cell Line
G0 Phase / physiology
G1 Phase / physiology
Kv1.4 Potassium Channel
Pituitary Gland / cytology*,  metabolism*,  physiology
Potassium Channels / metabolism*,  physiology
Potassium Channels, Voltage-Gated*
Shal Potassium Channels
Time Factors
Reg. No./Substance:
0/Kv1.4 Potassium Channel; 0/Potassium Channels; 0/Potassium Channels, Voltage-Gated; 0/Shal Potassium Channels

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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