Document Detail


Posttranslational modifications and activity of natural and recombinant tissue factor.
MedLine Citation:
PMID:  20138335     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue factor is a membrane protein, which in a complex with factor VIIa initiates in vivo blood coagulation. Due to the scarcity of natural tissue factor protein, most studies have relied upon recombinant tissue factor forms. However, there have been only cursory experimental comparisons of natural and recombinant tissue factor proteins. Our preliminary data suggested that placental tissue factor in a complex with factor VIIa was more efficient activator of factor X than the recombinant protein. After deglycosylation, both forms of tissue factor showed almost an identical activity in the extrinsic factor Xase. Analyses using tryptic digestion and mass-spectrometry revealed that the levels of glycosylation and the composition of carbohydrates present in natural placental tissue factor were different than those in its recombinant counterpart. These data indicate that natural and recombinant tissue factor proteins differ in their posttranslational modifications and that these differences translate into different cofactor activity. Thus the use of recombinant tissue factor proteins for the quantitation of natural tissue factor is misleading.
Authors:
Saulius Butenas; Jolanta Krudysz-Amblo; Kenneth G Mann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2010-02-06
Journal Detail:
Title:  Thrombosis research     Volume:  125 Suppl 1     ISSN:  1879-2472     ISO Abbreviation:  Thromb. Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-10-29     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  S26-8     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Biochemistry, University of Vermont, 208 South Park Drive, Burlington, VT 05446, USA. sbutenas@uvm.edu
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MeSH Terms
Descriptor/Qualifier:
Blood Coagulation
Carbohydrates / chemistry
Cell Line
Cell Membrane / metabolism
Factor VIIa / chemistry
Glycoproteins / chemistry
Glycosylation
Humans
Mannose / chemistry
Mass Spectrometry / methods
Molecular Weight
Protein Processing, Post-Translational*
Recombinant Proteins / chemistry*
Thromboplastin / chemistry*
Grant Support
ID/Acronym/Agency:
P01 HL046703-180007/HL/NHLBI NIH HHS; P01 HL46703/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Glycoproteins; 0/Recombinant Proteins; 31103-86-3/Mannose; 9035-58-9/Thromboplastin; EC 3.4.21.21/Factor VIIa
Comments/Corrections

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