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Posttranslational modification and microtubule stability.
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MedLine Citation:
PMID:  3316248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have probed the relationship between tubulin posttranslational modification and microtubule stability, using a variation of the antibody-blocking technique. In human retinoblastoma cells we find that acetylated and detyrosinated microtubules represent congruent subsets of the cells' total microtubules. We also find that stable microtubules defined as those that had not undergone polymerization within 1 h after injection of biotin-tubulin were all posttranslationally modified; furthermore dynamic microtubules were all unmodified. We therefore conclude that in these cells the stable, acetylated, and detyrosinated microtubules represent the same subset of the cells' total network. Posttranslational modification, however, is not a prerequisite for microtubule stability and vice versa. Potorous tridactylis kidney cells have no detectable acetylated microtubules but do have a sizable subset of stable ones, and chick embryo fibroblast cells are extensively modified but have few stable microtubules. We conclude that different cell types can create specific microtubule subsets by modulating the relative rates of posttranslational modification and microtubule turnover.
Authors:
E Schulze; D J Asai; J C Bulinski; M Kirschner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  105     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1988-01-07     Completed Date:  1988-01-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2167-77     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Brain / metabolism
Cattle
Cell Line
Chick Embryo
Eye Neoplasms
Fluorescent Antibody Technique
Humans
Microtubule Proteins / genetics*
Microtubules / ultrastructure*
Protein Processing, Post-Translational*
Retinoblastoma
Tubulin / metabolism
Chemical
Reg. No./Substance:
0/Antibodies; 0/Microtubule Proteins; 0/Tubulin
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1987
Volume: 105 Issue: 5
First Page: 2167 Last Page: 2177
ID: 2114866
Publisher Id: 88059249
PubMed Id: 3316248

Posttranslational modification and microtubule stability


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