| Posttranslational modification of interleukin-2 is a late event during activation of human T lymphocytes by ionophore A23187 and phorbol ester. | |
| | |
MedLine Citation:
|
PMID: 3122759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human peripheral blood lymphocytes secrete high titers of interleukin-2 (IL-2) after stimulation by Ca2+-ionophore A23187/phorbol 12-myristate-13-acetate. During the first 30 hours of incubation cells secrete only the nonglycosylated IL-2 M form of the lymphokine, the glycosylated forms IL-2 N1,2 being detected only after prolonged culture times (30-48 h). After recultivation of cells for a second 48 h period (without additional mitogen), the glycosylated and nonglycosylated IL-2 forms are secreted at a constant ratio of 7:3 throughout. The detection of glycosylated IL-2 is parallelled by an increase in cellular glycosyltransferase activities involved in formation of sialylated oligosaccharides O-linked to proteins. |
| | |
Authors:
|
H S Conradt; H Hauser; C Lorenz; H Mohr; A Plessing |
Related Documents
:
|
18761819 - Ex vivo stimulation of tumor-draining lymph node cells from lung cancer patients: a pot... 2600979 - Biologic characteristics of an immunosuppressive factor derived from a human lung cance... 2110979 - Pregnancy zone protein inhibits production of interleukin-2 but does not affect interle... 21533199 - Biphasic electrical currents stimulation promotes both proliferation and differentiatio... 21380689 - Evaluation of natural killer cell activity. 3496609 - Interleukin-2 induction, response and therapy on murine lupus lesions in the mrl/l strain. 7973459 - Human th0-type t helper-cell clone supports antigen-specific immunoglobulin production ... 22983579 - Plerixafor and g-csf for first-line steady-state autologous peripheral blood stem cell ... 18761819 - Ex vivo stimulation of tumor-draining lymph node cells from lung cancer patients: a pot... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 150 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1988 Jan |
Date Detail:
|
Created Date: 1988-02-20 Completed Date: 1988-02-20 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 97-103 Citation Subset: IM |
Affiliation:
|
Dept. of Genetics, GBF - Gesellschaft für Biotechnologische Forschung mbH., Braunschweig, F.R.G. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Calcimycin
/
pharmacology* Electrophoresis, Polyacrylamide Gel Glycosylation Humans Immunoenzyme Techniques Interleukin-2 / metabolism* Kinetics Lymphocyte Activation / drug effects* N-Acetylneuraminic Acid Protein Processing, Post-Translational* Sialic Acids / metabolism T-Lymphocytes / metabolism* Tetradecanoylphorbol Acetate / pharmacology* Transferases / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Interleukin-2; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid; 16561-29-8/Tetradecanoylphorbol Acetate; 52665-69-7/Calcimycin; EC 2.-/Transferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glu 46 of ribonuclease T1 is an essential residue for the recognition of guanine base.
Next Document: Alterations in fatty acyl composition can selectively affect amino acid transport in Saccharomyces c...