Document Detail

Posttranscriptional regulation of ATA2 transport during liver regeneration.
MedLine Citation:
PMID:  11095976     Owner:  NLM     Status:  MEDLINE    
The recent cloning of ATA2, a cDNA displaying characteristics identical to the System A transporter, has provided the first molecular tool for study of System A-mediated amino acid transport in liver. Despite the 233 +/- 9 and 472 +/- 11% increase in System A transport activity following partial hepatectomy at 6 and 12 h, respectively, the steady-state level of ATA2 mRNA did not show a corresponding marked increase. Examination of the kinetic properties of System A following partial hepatectomy revealed a K(m) of 0.26 +/- 0.04 mM which is consistent with the reported K(m) for ATA2. These results indicate that a System A transporter present in regenerating liver and ATA2 are identical, but that the increase in System A activity following partial hepatectomy does not result from an increase in steady-state levels of ATA2 mRNA. These observations suggest that ATA2-mediated transport of amino acids is regulated at the posttranscriptional level.
T L Freeman; M E Mailliard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  278     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-12     Completed Date:  2001-01-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  729-32     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA.
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MeSH Terms
Amino Acid Transport Systems
Amino Acids / metabolism
Biological Transport
Carrier Proteins / genetics*,  metabolism*
Gene Expression Regulation*
Liver / metabolism*
Liver Regeneration / physiology*
RNA Processing, Post-Transcriptional*
RNA, Messenger / genetics*,  metabolism
Rats, Sprague-Dawley
beta-Alanine / analogs & derivatives*,  pharmacokinetics
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/Amino Acids; 0/Carrier Proteins; 0/RNA, Messenger; 107-95-9/beta-Alanine; 19036-43-2/2,2-dimethyl-beta-alanine

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