Document Detail

Posttranscriptional lipopolysaccharide regulation of the lysozyme gene at processing of the primary transcript in myelomonocytic HD11 cells.
MedLine Citation:
PMID:  9590245     Owner:  NLM     Status:  MEDLINE    
Lysozyme is increasingly expressed in macrophages in inflammatory response to bacterial LPS. In this study, we investigated the mechanisms that control expression of the lysozyme gene in myelomonocytic HD11 cells activated by LPS. Nuclear run-on transcription assays showed that LPS caused a 15-fold increase in the transcription rate of the lysozyme gene. However, Northern analyses with lysozyme cDNA and intron sequences revealed that the LPS-induced increase in nuclear lysozyme transcripts greatly exceeded the increase in transcription rate. Furthermore, nuclear lysozyme transcripts in untreated cells with a t(1/2) of <10 min were more unstable than those accumulated in LPS-activated cells. We suggested, therefore, that the increased lysozyme expression following LPS treatment was largely due to a nuclear stabilization of the primary transcript. Interestingly, the increase in stability of the lysozyme primary transcript was accompanied by changes in nuclear processing including an increase in poly(A) tail length, which gradually shortened after entering the cytoplasm. The long lysozyme poly(A) tail, however, did not result in any increase in polysomal recruitment for translation or in stability of the cytoplasmic lysozyme mRNA.
R Goethe; L Phi-van
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  160     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-05-28     Completed Date:  1998-05-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4970-8     Citation Subset:  AIM; IM    
Institut für Tierzucht und Tierverhalten (FAL), Celle, Germany.
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MeSH Terms
Cell Line
Cell Nucleus / metabolism
Gene Expression Regulation / drug effects*
Lipopolysaccharides / pharmacology*
Muramidase / biosynthesis,  genetics*
RNA Precursors / metabolism
RNA Splicing
RNA, Messenger / metabolism*
Transcription, Genetic / drug effects
Reg. No./Substance:
0/Lipopolysaccharides; 0/RNA Precursors; 0/RNA, Messenger; EC

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