Document Detail

Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male mouse germ cells.
MedLine Citation:
PMID:  23284137     Owner:  NLM     Status:  MEDLINE    
The epigenetic status of germ cells changes dynamically during development. In this study, we analyzed the dynamics of histone H3 lysine 9 dimethylation (H3K9me2), a highly conserved mark of epigenetic silencing, and the expression of two lysine methyltransferases, G9a/Ehmt2/KMT1C and GLP/Ehmt1/KMT1D, in murine male embryonic germ cells after sex determination. Our previous studies established that G9a and GLP are the primary enzymes for H3K9me2 and predominantly exist as a G9a-GLP heteromeric complex that appears to be a functional H3K9 methyltransferase in vivo. During the period from Embryonic Day (E) 13.5 to E18.5 in mice, gonadal H3K9me2 levels were substantially lower in germ cells than in cells of nongerm lineage. Immunohistochemical analysis showed that during this phase in development, GLP level, but not G9a level, was also significantly lower in male germ cells. However, GLP mRNA was present in E13 and E16 male germ cells, with levels similar to those in cells of nongerm lineage. Interestingly, GLP is upregulated in embryonic male germ cells deficient for Nanos2, which encodes a germ cell-specific RNA-binding protein. Our data suggest that GLP protein expression is posttranscriptionally regulated in murine embryonic male germ cells after sex determination and that low H3K9me2 level results from the absence of GLP (severe reduction of the G9a-GLP heteromeric complex).
Katsuaki Deguchi; Go Nagamatsu; Hitoshi Miyachi; Yuzuru Kato; Sumiyo Morita; Hiroshi Kimura; Satsuki Kitano; Izuho Hatada; Yumiko Saga; Makoto Tachibana; Yoichi Shinkai
Related Documents :
23538447 - Apoptosis of osteosarcoma cultures by the combination of the cyclin-dependent kinase in...
25107587 - Mj-66 induces malignant glioma cells g2/m phase arrest and mitotic catastrophe through ...
23466887 - Accumulation of cadmium in insulin-producing β cells.
23412737 - Facile fabrication of co-sensitized plastic dye-sensitized solar cells using multiple e...
14514037 - Effect of agmatine against cell death induced by nmda and glutamate in neurons and pc12...
16122727 - Genetic determinants of pancreatic epsilon-cell development.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-02-14
Journal Detail:
Title:  Biology of reproduction     Volume:  88     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-02-15     Completed Date:  2013-08-12     Revised Date:  2013-08-19    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  36     Citation Subset:  IM    
Institute for Virus Research, Kyoto University, Kyoto, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Embryo, Mammalian / metabolism
Embryonic Development / physiology
Histone-Lysine N-Methyltransferase / genetics,  metabolism*
Mice, Inbred C57BL
Models, Animal
RNA Processing, Post-Transcriptional / physiology*
Spermatozoa / metabolism*
Reg. No./Substance:
EC protein, mouse; EC protein, mouse; EC N-Methyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Sternal traction: simple technique to prevent tamponade/compression of the heart.
Next Document:  Changes in eutopic endometrial gene expression during the progression of experimental endometriosis ...