Document Detail


Postprandial studies on dietary cholesterol in human subjects using stable isotopes and gas chromatography-mass spectrometry analysis.
MedLine Citation:
PMID:  9888648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We hypothesized that intestinal absorption and postprandial re-secretion of dietary cholesterol may be a particularly complex process in humans. To test this hypothesis, we used deuterium-enriched cholesterol to specifically label meal cholesterol and developed an improved method for quantitative measurement of traces of deuterated cholesterol as well as cholesterol with reference to two different internal standards by gas chromatography-mass spectrometry (GC-MS) measurement. In the first study, a group of healthy subjects ingested a single test meal containing deuterated cholesterol with a 7 h postprandial follow-up. In the second one, a group of healthy subjects ingested a first test meal containing deuterated cholesterol and a follow-up was performed during three consecutive test meals and later until 72 h. The most striking observations were that the occurrence of dietary cholesterol in chylomicrons is not concomitant to triglycerides and is very low after a single meal while most dietary cholesterol is re-secreted in chylomicrons after a second, and even a third, fat test meal. The data obtained show that the re-secretion of dietary cholesterol from the small intestine is a slow and complex process in humans.
Authors:
G Beaumier-Gallon; C Dubois; H Portugal; D Lairon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Atherosclerosis     Volume:  141 Suppl 1     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-03-16     Completed Date:  1999-03-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  S81-5     Citation Subset:  IM    
Affiliation:
Unit 476, Human Nutrition and Lipids at INSERM (National Institute of Health and Medical Research, Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cholesterol, Dietary / administration & dosage,  metabolism*
Chromatography, Gas
Deuterium
Humans
Male
Mass Spectrometry
Postprandial Period
Chemical
Reg. No./Substance:
0/Cholesterol, Dietary; 7782-39-0/Deuterium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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