| Postprandial increase of oleoylethanolamide mobilization in small intestine of the Burmese python (Python molurus). | |
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MedLine Citation:
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PMID: 16373434 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oleoylethanolamide (OEA) is an endogenous lipid mediator that inhibits feeding in rats and mice by activating the nuclear receptor peroxisome proliferator-activated receptor-alpha (PPAR-alpha). In rodents, intestinal OEA levels increase about threefold upon refeeding, a response that may contribute to the induction of between-meal satiety. Here, we examined whether feeding-induced OEA mobilization also occurs in Burmese pythons (Python molurus), a species of ambush-hunting snakes that consume huge meals after months of fasting and undergo massive feeding-dependent changes in gastrointestinal hormonal release and gut morphology. Using liquid chromatography/mass spectrometry (LC/MS), we measured OEA levels in the gastrointestinal tract of fasted (28 days) and fed (48 h after feeding) pythons. We observed a nearly 300-fold increase in OEA levels in the small intestine of fed compared with fasted animals (322 +/- 121 vs. 1 +/- 1 pmol/mg protein, n = 3-4). In situ OEA biosynthesis was suggested by the concomitant increase of N-acyl phosphatidylethanolamine species that serve as potential biosynthetic precursors for OEA. Furthermore, we observed a concomitant increase in saturated, mono- and diunsaturated, but not polyunsaturated fatty-acid ethanolamides (FAE) in the small intestine of fed pythons. The identification of OEA and other FAEs in the gastrointestinal tract of Python molurus suggests that this class of lipid messengers may be widespread among vertebrate groups and may represent an evolutionarily ancient means of regulating energy intake. |
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Authors:
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Giuseppe Astarita; Bryan C Rourke; Johnnie B Andersen; Jin Fu; Janet H Kim; Albert F Bennett; James W Hicks; Daniele Piomelli |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2005-12-22 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 290 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-04-10 Completed Date: 2006-05-31 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1407-12 Citation Subset: IM |
Affiliation:
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Dept. of Psychiatry and Human Behavior, Univ. of California, Irvine, CA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Boidae / metabolism* Colon / metabolism Fasting / metabolism Female Intestine, Small / metabolism* Lipid Metabolism / physiology Male Mass Spectrometry Oleic Acids / metabolism* Phosphatidylethanolamines / metabolism Phospholipids / metabolism Postprandial Period / physiology* Stomach / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK-070347/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oleic Acids; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/oleoylethanolamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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