| Postprandial Inhibition of Gastric Ghrelin Secretion by Long-chain Fatty Acid (LCFA) Through GPR 120 in Isolated Gastric Ghrelin Cells and Mice. | |
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MedLine Citation:
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PMID: 22678998 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Ghrelin is a gastric peptide hormone controlling appetite and energy homeostasis. Plasma ghrelin levels rise before a meal and fall quickly thereafter. Elucidation of the regulation of ghrelin secretion has been hampered by the difficulty of directly interrogating ghrelin cells diffusely scattered within the complex gastric mucosa. Therefore, we generated transgenic mice with ghrelin cell expression of green fluorescent protein (GFP) to enable characterization of ghrelin secretion in a pure population of isolated gastric ghrelin expressing GFP cells (Ghr-GFP cells). Using qRT-PCR and immunofluorescence staining, we detected a high level expression of the LCFA receptor GPR120, while the other LCFA receptor, GPR40, was undetectable. In short-term cultured pure Ghr-GFP cells, LCFA docosadienoic acid (DSA), linolenic acid (LLA), and palmitoleic acid (PTA) significantly suppressed ghrelin secretion. The physiological mechanism of LCFA inhibition on ghrelin secretion was studied in mice. Serum ghrelin levels were transiently suppressed after gastric gavage of LCFA-rich lipid in mice with pylorus ligation, indicating that the ghrelin cell may directly sense increased gastric LCFA derived from ingested intraluminal lipids. Meal-induced increase in gastric mucosal LCFA were assessed by measuring the transcripts of markers for tissue uptake of LCFA, lipoprotein lipase (LPL), fatty acid translocase (CD36), glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1), and nuclear fatty acid receptor PPARγ. QRT-PCR studies indicated significantly increased mRNA levels of LPL, GPIHBP1 and PPARγ in postprandial gastric mucosa. These results suggest that meal related increases in gastric mucosal LCFA interact with GPR120 on ghrelin cells to inhibit ghrelin secretion. |
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Authors:
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Xinping Lu; Xilin Zhao; Jianying Feng; Alice P Liou; Shari Anthony; Susanne Pechhold; Yuxiang Sun; Huiyan Lu; Stephen A Wank |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-7 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: - ISSN: 1522-1547 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1National Institutes of Health. |
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