Document Detail


Postnatal neural precursor cell regions in the rostral subventricular zone, hippocampal subgranular zone and cerebellum of the dog (Canis lupus familiaris).
MedLine Citation:
PMID:  23192285     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Identification of neural stem and progenitor cells (NPCs) in vitro and in vivo is essential to the use of developmental and disease models of neurogenesis. The dog is a valuable large animal model for multiple neurodegenerative diseases and is more closely matched to humans than rodents with respect to brain organization and complexity. It is therefore important to determine whether immunohistochemical markers associated with NPCs in humans and rodents are also appropriate for the dog. The NPC markers CD15, CD133, nestin, GFAP and phosphacan (DSD-1) were evaluated in situ in the canine rostral telencephalon, hippocampal dentate gyrus, and cerebellum at different postnatal time-points. Positive staining results were interpreted in the context of region and cellular morphology. Our results showed that neurospheres and cells within the rostral subventricular zone (SVZ), dentate gyrus subgranular zone (SGZ), and white matter tracts of the cerebellum were immunopositive for CD15, nestin and GFAP. Neurospheres and the cerebellum were immunonegative for CD133, whereas CD133 staining was present in the postnatal rostral SVZ. Anti-phosphacan antibody staining delineated the neurogenic niches of the rostral lateral ventricle SVZ and the hippocampal SGZ. Positive staining for phosphacan was also noted in white matter tracts of the cerebellum and within the Purkinje layer. Our results showed that in the dog these markers were associated with regions shown to be neurogenic in rodents and primates.
Authors:
Raquel M Walton; Thomas Parmentier; John H Wolfe
Related Documents :
10198205 - Unidirectional transport from apical to basolateral compartment of cobalt ion in polari...
19506035 - Mammalian fat and dachsous cadherins regulate apical membrane organization in the embry...
17114355 - Subcellular trafficking of the arabidopsis auxin influx carrier aux1 uses a novel pathw...
9452505 - Signal-dependent trafficking of beta-amyloid precursor protein-transferrin receptor chi...
9326175 - Cellular effects of hydroxyurea in hb sc disease.
6787115 - Immunohistochemical localization of galactosyltransferase in human fibroblasts and hela...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-29
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  -     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  HP-?-CD-PLGA nanoparticles improve the penetration and bioavailability of puerarin and enhance the t...
Next Document:  Objective and subjective oral health care needs among adults with various disabilities.