Document Detail


Postnatal maturation of gephyrin/glycine receptor clusters on developing Renshaw cells.
MedLine Citation:
PMID:  10980488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adult mammalian Renshaw cells express large and complex postsynaptic gephyrin/glycine receptor clusters on their surface. Larger gephyrin clusters correlate with more "efficacious" inhibitory synapses, in terms of larger postsynaptic quantal size amplitudes, in part because they likely contain more postsynaptic receptors (Lim et al. [1999] J. Physiol. (Lond.) 516:505-512; Oleskevich et al. [1999] J. Neurophysiology 82:312-319). Here, we studied the postnatal development of the gephyrin/glycine receptor cluster size on Renshaw cells. Renshaw cells were identified by their calbindin immunoreactivity, location and morphology, and presence of cholinergic input. The populations of clusters over developing Renshaw cells immunoreactive to gephyrin or glycine receptor alpha1 subunits were comparable in number, size, and complexity and displayed a high degree of colocalization (>90%) at all ages. Quantitative morphologic analysis was performed on gephyrin-immunoreactive clusters. In neonatal animals, Renshaw cells expressed small punctate gephyrin-immunoreactive clusters (mean cluster size +/- SD = 0.19 +/- 0.19 microm(2)at 2 days; 0.22 +/- 0. 19 microm(2)at 5 days). By 10 and 15 days of age, Renshaw cells exhibited gephyrin-immunoreactive clusters that were larger and more complex (0.32 +/- 0.19 microm(2) at 10 days; 0.41 +/- 0.32 microm(2) at 15 days). Cluster growth reached a plateau in 25- and 60-day-old Renshaw cells (0.45 +/- 0.43 microm(2); 0.56 +/- 0.55 microm(2), respectively). By using electron microscopy, we confirmed that gephyrin-immunoreactive clusters were located at postsynaptic sites at both early and late postnatal ages on Renshaw cells. The potential significance of this gephyrin/glycine receptor cluster size maturation that sets Renshaw cells apart from other interneurons is discussed.
Authors:
E J Geiman; M C Knox; F J Alvarez
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  426     ISSN:  0021-9967     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-16     Completed Date:  2000-10-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  130-42     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Wiley-Liss, Inc.
Affiliation:
Department of Anatomy, Wright State University, Dayton, Ohio 45435, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Calcium-Binding Protein, Vitamin D-Dependent / metabolism
Carrier Proteins / metabolism*
Cell Size / physiology
Female
Interneurons / metabolism*,  ultrastructure
Male
Membrane Proteins / metabolism*
Rats
Rats, Sprague-Dawley
Receptors, Glycine / metabolism*
Spinal Cord / growth & development*,  metabolism,  ultrastructure
Synaptic Membranes / metabolism,  ultrastructure
Grant Support
ID/Acronym/Agency:
NS 33555/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Carrier Proteins; 0/Membrane Proteins; 0/Receptors, Glycine; 0/calbindin; 0/gephyrin

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