| Postnatal development of lipopolysaccharide-induced inflammatory response in the brain. | |
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MedLine Citation:
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PMID: 20865294 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: This study aimed to characterize postnatal development of lipopolysaccharide (LPS)-induced inflammatory response in the brain. METHODS: Postnatal day (P)1, P21 and P70 Sprague-Dawley(®) rats were treated with saline or 0.25 mg/kg LPS for 2 h, and the mRNA expression of neuroinflammatory mediators in the brain was determined using reverse transcriptase-polymerase chain reaction (RT-PCR). The kinetics of LPS-induced neuroinflammatory mediators in the brain of P1 and P21 animals was determined using RT-PCR, and the kinetics of LPS-induced cytokines in the serum were determined using ELISA. The basal levels of Toll-like receptor (TLR)-4, CD14, and myeloid differentiation factor 88 (Myd88) were measured at the mRNA and protein levels using RT-PCR and Western blot assay respectively. RESULTS: The mRNA expression levels of cytokines and chemokines were considerably increased in P21 and P70 brains but not significantly altered in P1 brain at 2 h following LPS stimulation. Instead, the induction of cytokines and chemokines was significantly delayed in the brain of P1 animals following LPS stimulation, which was associated with diminished Myd88 production in P1 brain. In parallel, the cytokine response in the serum of P1 animals after LPS stimulation was also delayed compared to P21 animals. CONCLUSIONS: TLR-4-mediated innate immunity in the brain was significantly delayed in P1 animals, and underwent significant development during the early postnatal period. |
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Authors:
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Andres Ortega; Viren Jadeja; Heping Zhou |
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Publication Detail:
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Type: Journal Article Date: 2010-09-24 |
Journal Detail:
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Title: Inflammation research : official journal of the European Histamine Research Society ... [et al.] Volume: 60 ISSN: 1420-908X ISO Abbreviation: Inflamm. Res. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508160 Medline TA: Inflamm Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 175-85 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Seton Hall University, 400 South Orange Avenue, South Orange, NJ, 07079, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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