Document Detail


Postnatal development- and age-related changes in DNA-methylation patterns in the human genome.
MedLine Citation:
PMID:  22495928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alterations in DNA methylation have been reported to occur during development and aging; however, much remains to be learned regarding post-natal and age-associated epigenome dynamics, and few if any investigations have compared human methylome patterns on a whole genome basis in cells from newborns and adults. The aim of this study was to reveal genomic regions with distinct structure and sequence characteristics that render them subject to dynamic post-natal developmental remodeling or age-related dysregulation of epigenome structure. DNA samples derived from peripheral blood monocytes and in vitro differentiated dendritic cells were analyzed by methylated DNA Immunoprecipitation (MeDIP) or, for selected loci, bisulfite modification, followed by next generation sequencing. Regions of interest that emerged from the analysis included tandem or interspersed-tandem gene sequence repeats (PCDHG, FAM90A, HRNR, ECEL1P2), and genes with strong homology to other family members elsewhere in the genome (FZD1, FZD7 and FGF17). Our results raise the possibility that selected gene sequences with highly homologous copies may serve to facilitate, perhaps even provide a clock-like function for, developmental and age-related epigenome remodeling. If so, this would represent a fundamental feature of genome architecture in higher eukaryotic organisms.
Authors:
Paraskevi Salpea; Valya R Russanova; Tazuko H Hirai; Thomae G Sourlingas; Kalliope E Sekeri-Pataryas; Roberto Romero; Jonathan Epstein; Bruce H Howard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2012-04-11
Journal Detail:
Title:  Nucleic acids research     Volume:  40     ISSN:  1362-4962     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-08     Completed Date:  2012-11-05     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  6477-94     Citation Subset:  IM    
Affiliation:
Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aging / genetics*
Cells, Cultured
DNA Methylation*
Female
Genes
Genome, Human*
Humans
Immunoprecipitation
Infant, Newborn
Male
Multigene Family
Sequence Analysis, DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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