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Postnatal development and LPS-responsiveness of pulmonary adenosine receptor expression and of adenosine-metabolizing enzymes in mice.
MedLine Citation:
PMID:  25188742     Owner:  NLM     Status:  Publisher    
Background:Adenosine levels are regulated by ecto-5'-nucleotidase/CD73 and adenosine deaminase (ADA). Adenosine regulates endothelial permeability and anti-inflammatory responses via adenosine receptors. Here, the adenosine receptors and purine-converting enzymes were studied during postnatal development and inflammation.Methods:Newborn, 1-, 10-, 14-day-old and adult C57BL/6 mice were challenged i.p. with LPS for six hours. The inflammatory response was evaluated by histochemistry. Expression levels of adenosine receptors (A1, A2A, A2B and A3), CD73 and ADA were measured by qRT-PCR. A1 was studied by immunohistochemistry and enzyme activities were analyzed by thin-layer chromatography.Results:LPS caused respiratory distress in newborns within 24 hours. LPS induced neutrophils at the basal stage and alveolar congestion. Low activity and expression of CD73 increased after birth. Expression of ADA after LPS increased 16-fold in adults and 2-fold in newborns (p<0.05). A1 expression was high in newborns and increased after LPS (p<0.05). A1 was localized to endothelial membranes. A2A decreased after LPS in newborns and increased in adults (p<0.05). The expression of A3 increased in newborns and adults after LPS.Conclusion:Low pulmonary CD73 expression, LPS-induced suppression of A2A, LPS-induced increase of A1 expression and severe respiratory distress were distinguishing responses in the newborns from those in the adults.Pediatric Research (2014); doi:10.1038/pr.2014.132.
Juhani Metsola; Mikael Maksimow; Marja Ojaniemi; Hanna Metsola; Fumiko Marttila-Ichihara; Reetta Vuolteenaho; Gennady G Yegutkin; Marko Salmi; Mikko Hallman; Sirpa Jalkanen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-04
Journal Detail:
Title:  Pediatric research     Volume:  -     ISSN:  1530-0447     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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