Document Detail


Postnatal amniotic fluid intake reduces gut inflammatory responses and necrotizing enterocolitis in preterm neonates.
MedLine Citation:
PMID:  23518680     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Preterm neonates are susceptible to gastrointestinal disorders such as necrotizing enterocolitis (NEC). Maternal milk and colostrum protects against NEC via growth promoting, immunomodulatory, and antimicrobial factors. The fetal enteral diet, amniotic fluid (AF), contains similar components, and we hypothesized that postnatal AF administration reduces inflammatory responses and NEC in preterms neonates. Preterm pigs (92% gestation) were delivered by caesarean section and fed parental nutrition (2 d) followed by enteral (2 d) porcine colostrum (COLOS, n = 7), infant formula (FORM, n = 13), or AF supplied before and after introduction of formula (AF, n = 10) in Experiment 1, and supplied only during the enteral feeding period in Experiment 2 (FORM, n = 16; AF, n = 14). The NEC score was reduced in both AF and COLOS pigs, relative to FORM when AF was provided prior to full enteral feeding (9.9 and 7.7 compared with 17.3, P < 0.05). There was no effect of AF when provided only during enteral feeding. AF pigs showed decreased bacterial abundance in colon and intestinal inflammation-related genes (e.g. TNF-α, IL-1α, IL-6, NOS) were down-regulated, relative to FORM pigs with NEC. Anti-inflammatory properties of AF were supported by delayed maturation and decreased TNF-α production in murine dendritic cells, as well as increased proliferation and migration, and down-regulation of IL-6 expression in intestinal cells (IEC-6, IPEC-J2). Like colostrum, AF may reduce NEC development in preterm neonates by suppressing the pro-inflammatory responses to enteral formula feeding and gut colonization when provided before the onset of NEC.
Authors:
Jayda L Siggers; Mette Viberg Ostergaard; Richard H Siggers; Kerstin Skovgaard; Lars Mølbak; Thomas Thymann; Mette Schmidt; Hanne Kristine Møller; Stig Purup; Lisbeth Nielsen Fink; Hanne Frøkiær; Mette Boye; Per T Sangild; Stine Brandt Bering
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-3-21
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  -     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-3-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1University of Copenhagen.
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